biostudies-literatureGourguechon SPHS HHS142-5https://www.ebi.ac.uk/biostudies/studies/S-EPMC3019802biostudies-literatureUnknown10(1)We developed a series of plasmids that allow C-terminal tagging of any gene in its endogenous locus in Giardia intestinalis, with different epitope tags (triple hemagglutinin [3HA] and triple Myc [3Myc]) and selection markers (puromycin, neomycin, and a newly developed marker, blasticidin). Using these vectors, cyclin B and aurora kinase were tagged, expressed, and localized.Eukaryotic cellRapid tagging and integration of genes in Giardia intestinalis.PMC3019802A1054693Gourguechon SCande WZfalseRapid tagging and integration of genes in Giardia intestinalis.We developed a series of plasmids that allow C-terminal tagging of any gene in its endogenous locus in Giardia intestinalis, with different epitope tags (triple hemagglutinin [3HA] and triple Myc [3Myc]) and selection markers (puromycin, neomycin, and a newly developed marker, blasticidin). Using these vectors, cyclin B and aurora kinase were tagged, expressed, and localized.2011-01-01T00:00:00Z2011 Jan2025-04-22T20:58:42.384Z2019-03-27T00:38:01ZS-EPMC30198022111573910.1128/EC.00190-1010.1128/ec.00190-10