{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Xu C"],"funding":["NIAAA NIH HHS"],"pagination":["449-59"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3019819"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["193(2)"],"pubmed_abstract":["The O antigen is the outer part of the lipopolysaccharide (LPS) in the outer membrane of Gram-negative bacteria and contains many repeats of an oligosaccharide unit. It contributes to antigenic variability and is essential to the full function and virulence of bacteria. Shigella is a Gram-negative human pathogen that causes diarrhea in humans. The O antigen of Shigella boydii type 14 consists of repeating oligosaccharide units with the structure [→6-d-Galpα1→4-d-GlcpAβ1→6-d-Galpβ1→4-d-Galpβ1→4-d-GlcpNAcβ1→]n. The wfeD gene in the O-antigen gene cluster of Shigella boydii type 14 was proposed to encode a galactosyltransferase (GalT) involved in O-antigen synthesis. We confirmed here that the wfeD gene product is a β4-GalT that synthesizes the Galβ1-4GlcNAcα-R linkage. WfeD was expressed in "],"journal":["Journal of bacteriology"],"pubmed_title":["Biochemical characterization of UDP-Gal:GlcNAc-pyrophosphate-lipid β-1,4-Galactosyltransferase WfeD, a new enzyme from Shigella boydii type 14 that catalyzes the second step in O-antigen repeating-unit synthesis."],"pmcid":["PMC3019819"],"funding_grant_id":["R01 AA020703"],"pubmed_authors":["Allingham JS","Feng L","Szarek WA","Xu C","Liu B","Han Y","Hu B","Wang L","Brockhausen I"],"additional_accession":[]},"is_claimable":false,"name":"Biochemical characterization of UDP-Gal:GlcNAc-pyrophosphate-lipid β-1,4-Galactosyltransferase WfeD, a new enzyme from Shigella boydii type 14 that catalyzes the second step in O-antigen repeating-unit synthesis.","description":"The O antigen is the outer part of the lipopolysaccharide (LPS) in the outer membrane of Gram-negative bacteria and contains many repeats of an oligosaccharide unit. It contributes to antigenic variability and is essential to the full function and virulence of bacteria. Shigella is a Gram-negative human pathogen that causes diarrhea in humans. The O antigen of Shigella boydii type 14 consists of repeating oligosaccharide units with the structure [→6-d-Galpα1→4-d-GlcpAβ1→6-d-Galpβ1→4-d-Galpβ1→4-d-GlcpNAcβ1→]n. The wfeD gene in the O-antigen gene cluster of Shigella boydii type 14 was proposed to encode a galactosyltransferase (GalT) involved in O-antigen synthesis. We confirmed here that the wfeD gene product is a β4-GalT that synthesizes the Galβ1-4GlcNAcα-R linkage. WfeD was expressed in ","dates":{"release":"2011-01-01T00:00:00Z","publication":"2011 Jan","modification":"2025-04-22T20:58:55.27Z","creation":"2019-03-27T00:38:02Z"},"accession":"S-EPMC3019819","cross_references":{"pubmed":["21057010"],"doi":["10.1128/JB.00737-10","10.1128/jb.00737-10"]}}