biostudies-literatureUnknown79(1)Campoy EMCoxiella burnetii is a Gram-negative obligate intracellular bacterium. After internalization, this bacterium replicates in a large parasitophorous vacuole that has features of both phagolysosomes and autophagosomal compartments. We have previously demonstrated that early after internalization Coxiella phagosomes interact with both the endocytic and the autophagic pathways. In this report, we present evidence that the Coxiella-replicative vacuoles (CRVs) also interact with the secretory pathway. Rab1b is a small GTPase responsible for the anterograde transport between the endoplasmic reticulum and the Golgi apparatus. We present evidence that Rab1b is recruited to the CRV at later infection times (i.e., after 6 h of infection). Interestingly, knockdown of Rab1b altered vacuole growth, indicInfection and immunity402-13https://www.ebi.ac.uk/biostudies/studies/S-EPMC3019900biostudies-literatureThe early secretory pathway contributes to the growth of the Coxiella-replicative niche.PMC3019900Zoppino FCCampoy EMColombo MIfalseThe early secretory pathway contributes to the growth of the Coxiella-replicative niche.Coxiella burnetii is a Gram-negative obligate intracellular bacterium. After internalization, this bacterium replicates in a large parasitophorous vacuole that has features of both phagolysosomes and autophagosomal compartments. We have previously demonstrated that early after internalization Coxiella phagosomes interact with both the endocytic and the autophagic pathways. In this report, we present evidence that the Coxiella-replicative vacuoles (CRVs) also interact with the secretory pathway. Rab1b is a small GTPase responsible for the anterograde transport between the endoplasmic reticulum and the Golgi apparatus. We present evidence that Rab1b is recruited to the CRV at later infection times (i.e., after 6 h of infection). Interestingly, knockdown of Rab1b altered vacuole growth, indic2011-01-01T00:00:00Z2011 Jan2025-04-22T05:13:51.479Z2019-03-27T00:38:02ZS-EPMC30199002093776510.1128/IAI.00688-1010.1128/iai.00688-10