biostudies-literatureUnknown104(2)Matsuda R<h4>Background</h4>The aim of this study is to find a novel molecular target based on chromosomal alteration and array-based gene expression analyses in bladder cancer (BC). We investigated a cancer testis antigen, LY6K, which is located on chromosome 8q24.3.<h4>Methods</h4>Five BC cell lines were subjected to high-resolution array-comparative genomic hybridisation with 244 000 probes. The expression levels of LY6K mRNA were evaluated in BC cell lines and clinical BC specimens by real-time reverse transcription-PCR. The cell lines were subjected to fluorescence in situ hybridisation of LY6K. Cell viability was evaluated by cell growth, wound healing, and matrigel invasion assays.<h4>Results</h4>Typical gained loci (P<0.0001) at 6p21.33-p21.32, 8q24.3, 9q34.13, 11q13.1-q14.1, 12q13.12-q13.1British journal of cancer376-86https://www.ebi.ac.uk/biostudies/studies/S-EPMC3031884biostudies-literatureLY6K is a novel molecular target in bladder cancer on basis of integrate genome-wide profiling.PMC3031884Nakagawa MToki KTatarano SSugimoto TMatsuda RKawakami KYoshino HKawahara KChiyomaru TNishiyama KEnokida HKikkawa NSeki NUchida YfalseLY6K is a novel molecular target in bladder cancer on basis of integrate genome-wide profiling.<h4>Background</h4>The aim of this study is to find a novel molecular target based on chromosomal alteration and array-based gene expression analyses in bladder cancer (BC). We investigated a cancer testis antigen, LY6K, which is located on chromosome 8q24.3.<h4>Methods</h4>Five BC cell lines were subjected to high-resolution array-comparative genomic hybridisation with 244 000 probes. The expression levels of LY6K mRNA were evaluated in BC cell lines and clinical BC specimens by real-time reverse transcription-PCR. The cell lines were subjected to fluorescence in situ hybridisation of LY6K. Cell viability was evaluated by cell growth, wound healing, and matrigel invasion assays.<h4>Results</h4>Typical gained loci (P<0.0001) at 6p21.33-p21.32, 8q24.3, 9q34.13, 11q13.1-q14.1, 12q13.12-q13.12011-01-01T00:00:00Z2011 Jan2025-04-03T22:18:30.409Z2019-03-27T00:38:34ZS-EPMC30318842106339710.1038/sj.bjc.6605990