{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Piro JR"],"funding":["NIAID NIH HHS","NINDS NIH HHS"],"pagination":["e1002001"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3033378"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7(2)"],"pubmed_abstract":["Single-stranded polyanions ≥40 bases in length facilitate the formation of hamster scrapie prions in vitro, and polyanions co-localize with PrP(Sc) aggregates in vivo. To test the hypothesis that intact polyanionic molecules might serve as a structural backbone essential for maintaining the infectious conformation(s) of PrP(Sc), we produced synthetic prions using a photocleavable, 100-base oligonucleotide (PC-oligo). In serial Protein Misfolding Cyclic Amplification (sPMCA) reactions using purified PrP(C) substrate, PC-oligo was incorporated into physical complexes with PrP(Sc) molecules that were resistant to benzonase digestion. Exposure of these nuclease-resistant prion complexes to long wave ultraviolet light (315 nm) induced degradation of PC-oligo into 5 base fragments. Light-induced"],"journal":["PLoS pathogens"],"pubmed_title":["In situ photodegradation of incorporated polyanion does not alter prion infectivity."],"pmcid":["PMC3033378"],"funding_grant_id":["R01 NS046478","T32 AI007519","R01 NS055875","2R01 NS046478"],"pubmed_authors":["Harris BT","Supattapone S","Piro JR"],"additional_accession":[]},"is_claimable":false,"name":"In situ photodegradation of incorporated polyanion does not alter prion infectivity.","description":"Single-stranded polyanions ≥40 bases in length facilitate the formation of hamster scrapie prions in vitro, and polyanions co-localize with PrP(Sc) aggregates in vivo. To test the hypothesis that intact polyanionic molecules might serve as a structural backbone essential for maintaining the infectious conformation(s) of PrP(Sc), we produced synthetic prions using a photocleavable, 100-base oligonucleotide (PC-oligo). In serial Protein Misfolding Cyclic Amplification (sPMCA) reactions using purified PrP(C) substrate, PC-oligo was incorporated into physical complexes with PrP(Sc) molecules that were resistant to benzonase digestion. Exposure of these nuclease-resistant prion complexes to long wave ultraviolet light (315 nm) induced degradation of PC-oligo into 5 base fragments. Light-induced","dates":{"release":"2011-01-01T00:00:00Z","publication":"2011 Feb","modification":"2026-07-15T10:34:14.274Z","creation":"2026-07-03T03:12:19.863Z"},"accession":"S-EPMC3033378","cross_references":{"pubmed":["21304885"],"doi":["10.1371/journal.ppat.1002001"]}}