biostudies-literatureTeixeira FRNIGMS NIH HHS2746-57https://www.ebi.ac.uk/biostudies/studies/S-EPMC3034404biostudies-literatureUnknown10(15)FBXO25 is one of the 68 human F-box proteins that serve as specificity factors for a family of ubiquitin ligases composed of s-phase-kinase associated protein 1, really interesting new gene-box 1, Cullin 1, and F-box protein (SCF1) that are involved in targeting proteins for destruction across the ubiquitin proteasome system. We recently reported that the FBXO25 protein accumulates in novel subnuclear structures named FBXO25-associated nuclear domains (FAND). Combining two-step affinity purification followed by MS with a classical two-hybrid screen, we identified 132 novel potential FBXO25 interacting partners. One of the identified proteins, beta-actin, physically interacts through its N-terminus with FBXO25 and is enriched in the FBXO25 nuclear compartments. Inhibitors of actin polymerizProteomicsIdentification of FBXO25-interacting proteins using an integrated proteomics approach.PMC3034404R01 GM080587GM 080587R01 GM080587-01A2Teixeira FRKobarg JGartner CAManfiolli AOYokoo SBaqui MMde Lanerolle PGygi SPAssmann EMMaragno ALYu HGomes MDfalseIdentification of FBXO25-interacting proteins using an integrated proteomics approach.FBXO25 is one of the 68 human F-box proteins that serve as specificity factors for a family of ubiquitin ligases composed of s-phase-kinase associated protein 1, really interesting new gene-box 1, Cullin 1, and F-box protein (SCF1) that are involved in targeting proteins for destruction across the ubiquitin proteasome system. We recently reported that the FBXO25 protein accumulates in novel subnuclear structures named FBXO25-associated nuclear domains (FAND). Combining two-step affinity purification followed by MS with a classical two-hybrid screen, we identified 132 novel potential FBXO25 interacting partners. One of the identified proteins, beta-actin, physically interacts through its N-terminus with FBXO25 and is enriched in the FBXO25 nuclear compartments. Inhibitors of actin polymeriz2010-01-01T00:00:00Z2010 Aug2025-04-22T05:07:43.654Z2019-03-27T00:38:44ZS-EPMC30344042047397010.1002/pmic.200900419