{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hong L"],"funding":["NIEHS NIH HHS"],"pagination":["1101-9"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3046028"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["54(5)"],"pubmed_abstract":["Para-aminosalicylic acid (PAS), an approved drug for treatment of tuberculosis, is a promising therapeutic agent for treatment of manganese (Mn)-induced parkinsonian syndromes. Lack of a quantifying method, however, has hindered the clinical evaluation of its efficacy and there upon new drug development. This study was aimed at developing a simple and effective method to quantify PAS and its major metabolite, N-acetyl-para-aminosalicylic acid (AcPAS), in plasma, cerebrospinal fluid (CSF) and tissues. Biological samples underwent one-step protein precipitation. The supernatant was fractionated on a reversed-phase C18 column with a gradient mobile system, followed by on-line fluorescence detection. The lower limits of quantification for both PAS and AcPAS were 50 ng/ml of plasma and 17 ng/g "],"journal":["Journal of pharmaceutical and biomedical analysis"],"pubmed_title":["HPLC analysis of para-aminosalicylic acid and its metabolite in plasma, cerebrospinal fluid and brain tissues."],"pmcid":["PMC3046028"],"funding_grant_id":["R01ES008146","R01 ES008146-14","R01 ES008146"],"pubmed_authors":["Hong L","Zeng S","Jiang W","Zheng W"],"additional_accession":[]},"is_claimable":false,"name":"HPLC analysis of para-aminosalicylic acid and its metabolite in plasma, cerebrospinal fluid and brain tissues.","description":"Para-aminosalicylic acid (PAS), an approved drug for treatment of tuberculosis, is a promising therapeutic agent for treatment of manganese (Mn)-induced parkinsonian syndromes. Lack of a quantifying method, however, has hindered the clinical evaluation of its efficacy and there upon new drug development. This study was aimed at developing a simple and effective method to quantify PAS and its major metabolite, N-acetyl-para-aminosalicylic acid (AcPAS), in plasma, cerebrospinal fluid (CSF) and tissues. Biological samples underwent one-step protein precipitation. The supernatant was fractionated on a reversed-phase C18 column with a gradient mobile system, followed by on-line fluorescence detection. The lower limits of quantification for both PAS and AcPAS were 50 ng/ml of plasma and 17 ng/g ","dates":{"release":"2011-01-01T00:00:00Z","publication":"2011 Apr","modification":"2025-04-04T21:04:02.276Z","creation":"2019-03-27T00:39:15Z"},"accession":"S-EPMC3046028","cross_references":{"pubmed":["21159459"],"doi":["10.1016/j.jpba.2010.11.031"]}}