{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang M"],"funding":["NIGMS NIH HHS"],"pagination":["1811-22"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3061060"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["39(5)"],"pubmed_abstract":["Ribosome biogenesis requires multiple nuclease activities to process pre-rRNA transcripts into mature rRNA species and eliminate defective products of transcription and processing. We find that in mammalian cells, the 5' exonuclease Xrn2 plays a major role in both maturation of rRNA and degradation of a variety of discarded pre-rRNA species. Precursors of 5.8S and 28S rRNAs containing 5' extensions accumulate in mouse cells after siRNA-mediated knockdown of Xrn2, indicating similarity in the 5'-end maturation mechanisms between mammals and yeast. Strikingly, degradation of many aberrant pre-rRNA species, attributed mainly to 3' exonucleases in yeast studies, occurs 5' to 3' in mammalian cells and is mediated by Xrn2. Furthermore, depletion of Xrn2 reveals pre-rRNAs derived by cleavage even"],"journal":["Nucleic acids research"],"pubmed_title":["5'-end surveillance by Xrn2 acts as a shared mechanism for mammalian pre-rRNA maturation and decay."],"pmcid":["PMC3061060"],"funding_grant_id":["R01 GM074091-06","GM074091","R01 GM074091"],"pubmed_authors":["Wang M","Pestov DG"],"additional_accession":[]},"is_claimable":false,"name":"5'-end surveillance by Xrn2 acts as a shared mechanism for mammalian pre-rRNA maturation and decay.","description":"Ribosome biogenesis requires multiple nuclease activities to process pre-rRNA transcripts into mature rRNA species and eliminate defective products of transcription and processing. We find that in mammalian cells, the 5' exonuclease Xrn2 plays a major role in both maturation of rRNA and degradation of a variety of discarded pre-rRNA species. Precursors of 5.8S and 28S rRNAs containing 5' extensions accumulate in mouse cells after siRNA-mediated knockdown of Xrn2, indicating similarity in the 5'-end maturation mechanisms between mammals and yeast. Strikingly, degradation of many aberrant pre-rRNA species, attributed mainly to 3' exonucleases in yeast studies, occurs 5' to 3' in mammalian cells and is mediated by Xrn2. Furthermore, depletion of Xrn2 reveals pre-rRNAs derived by cleavage even","dates":{"release":"2011-01-01T00:00:00Z","publication":"2011 Mar","modification":"2025-04-19T21:40:14.878Z","creation":"2019-03-27T00:39:55Z"},"accession":"S-EPMC3061060","cross_references":{"pubmed":["21036871"],"doi":["10.1093/nar/gkq1050"]}}