biostudies-literatureUnknown104(7)Forster JACancer Research UK<h4>Background</h4>Therapies targeting ERBB2 have shown success in the clinic. However, response is not determined solely by expression of ERBB2. Levels of ERBB3, its preferred heterodimerisation partner and ERBB ligands may also have a role.<h4>Methods</h4>We measured NRG1 expression by real-time quantitative RT-PCR and ERBB receptors by western blotting and immunohistochemistry in bladder tumours and cell lines.<h4>Results</h4>NRG1α and NRG1β showed significant coordinate expression. NRG1β was upregulated in 78% of cell lines. In tumours, there was a greater range of expression with a trend towards increased NRG1α with higher stage and grade. Increased expression of ERBB proteins was detected in 15% (EGFR), 20% (ERBB2), 41% (ERBB3) and 0% (ERBB4) of cell lines. High EGFR expression was dBritish journal of cancer1135-43https://www.ebi.ac.uk/biostudies/studies/S-EPMC3068491biostudies-literatureExpression of NRG1 and its receptors in human bladder cancer.PMC3068491Paul ABHarnden PKnowles MAForster JAfalseExpression of NRG1 and its receptors in human bladder cancer.<h4>Background</h4>Therapies targeting ERBB2 have shown success in the clinic. However, response is not determined solely by expression of ERBB2. Levels of ERBB3, its preferred heterodimerisation partner and ERBB ligands may also have a role.<h4>Methods</h4>We measured NRG1 expression by real-time quantitative RT-PCR and ERBB receptors by western blotting and immunohistochemistry in bladder tumours and cell lines.<h4>Results</h4>NRG1α and NRG1β showed significant coordinate expression. NRG1β was upregulated in 78% of cell lines. In tumours, there was a greater range of expression with a trend towards increased NRG1α with higher stage and grade. Increased expression of ERBB proteins was detected in 15% (EGFR), 20% (ERBB2), 41% (ERBB3) and 0% (ERBB4) of cell lines. High EGFR expression was d2011-01-01T00:00:00Z2011 Mar2026-07-15T13:40:34.763Z2019-03-27T00:40:16ZS-EPMC30684912136458010.1038/bjc.2011.39