{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tottey S"],"funding":["NIGMS NIH HHS"],"pagination":["16119-24"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC307702"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["100(26)"],"pubmed_abstract":["CHL27, the Arabidopsis homologue to Chlamydomonas Crd1, a plastid-localized putative diiron protein, is required for the synthesis of protochlorophyllide and therefore is a candidate subunit of the aerobic cyclase in chlorophyll biosynthesis. delta-Aminolevulinic acid-fed antisense Arabidopsis plants with reduced amounts of Crd1/CHL27 accumulate Mg-protoporphyrin IX monomethyl ester, the substrate of the cyclase reaction. Mutant plants have chlorotic leaves with reduced abundance of all chlorophyll proteins. Fractionation of Arabidopsis chloroplast membranes shows that Crd1/CHL27 is equally distributed on a membrane-weight basis in the thylakoid and inner-envelope membranes."],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Arabidopsis CHL27, located in both envelope and thylakoid membranes, is required for the synthesis of protochlorophyllide."],"pmcid":["PMC307702"],"funding_grant_id":["R01 GM042143","R37 GM042143","GM42143"],"pubmed_authors":["Merchant S","Westergren T","Scheller HV","Allen M","Jensen PE","Tottey S","Block MA","Albrieux C"],"additional_accession":[]},"is_claimable":false,"name":"Arabidopsis CHL27, located in both envelope and thylakoid membranes, is required for the synthesis of protochlorophyllide.","description":"CHL27, the Arabidopsis homologue to Chlamydomonas Crd1, a plastid-localized putative diiron protein, is required for the synthesis of protochlorophyllide and therefore is a candidate subunit of the aerobic cyclase in chlorophyll biosynthesis. delta-Aminolevulinic acid-fed antisense Arabidopsis plants with reduced amounts of Crd1/CHL27 accumulate Mg-protoporphyrin IX monomethyl ester, the substrate of the cyclase reaction. Mutant plants have chlorotic leaves with reduced abundance of all chlorophyll proteins. Fractionation of Arabidopsis chloroplast membranes shows that Crd1/CHL27 is equally distributed on a membrane-weight basis in the thylakoid and inner-envelope membranes.","dates":{"release":"2003-01-01T00:00:00Z","publication":"2003 Dec","modification":"2024-11-08T19:05:20.042Z","creation":"2019-03-27T00:49:51Z"},"accession":"S-EPMC307702","cross_references":{"pubmed":["14673103"],"doi":["10.1073/pnas.2136793100"]}}