biostudies-literatureLai MNCI NIH HHSNINDS NIH HHS776-85https://www.ebi.ac.uk/biostudies/studies/S-EPMC3086669biostudies-literatureUnknown9(8)<h4>Background</h4>Voltage-gated potassium channels are thought to be the target of antibodies associated with limbic encephalitis. However, antibody testing using cells expressing voltage-gated potassium channels is negative; hence, we aimed to identify the real autoantigen associated with limbic encephalitis.<h4>Methods</h4>We analysed sera and CSF of 57 patients with limbic encephalitis and antibodies attributed to voltage-gated potassium channels and 148 control individuals who had other disorders with or without antibodies against voltage-gated potassium channels. Immunohistochemistry, immunoprecipitation, and mass spectrometry were used to characterise the antigen. An assay with HEK293 cells transfected with leucine-rich, glioma-inactivated 1 (LGI1) and disintegrin and metalloproteinThe Lancet. NeurologyInvestigation of LGI1 as the antigen in limbic encephalitis previously attributed to potassium channels: a case series.PMC3086669RC1 NS0682041RC1NS068204-01R01 CA089054R01 NS046706R01 NS077851R01 CA107192R01 CA089054-02R01CA107192NS046706Huijbers MGCowell JKDalmau JLancaster EGraus FBataller LLai MBalice-Gordon RfalseInvestigation of LGI1 as the antigen in limbic encephalitis previously attributed to potassium channels: a case series.<h4>Background</h4>Voltage-gated potassium channels are thought to be the target of antibodies associated with limbic encephalitis. However, antibody testing using cells expressing voltage-gated potassium channels is negative; hence, we aimed to identify the real autoantigen associated with limbic encephalitis.<h4>Methods</h4>We analysed sera and CSF of 57 patients with limbic encephalitis and antibodies attributed to voltage-gated potassium channels and 148 control individuals who had other disorders with or without antibodies against voltage-gated potassium channels. Immunohistochemistry, immunoprecipitation, and mass spectrometry were used to characterise the antigen. An assay with HEK293 cells transfected with leucine-rich, glioma-inactivated 1 (LGI1) and disintegrin and metalloprotein2010-01-01T00:00:00Z2010 Aug2025-04-18T18:56:55.192Z2019-03-27T00:41:12ZS-EPMC30866692058061510.1016/s1474-4422(10)70137-x10.1016/S1474-4422(10)70137-X