{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Spadafora ND"],"funding":["Biotechnology and Biological Sciences Research Council"],"pagination":["1183-92"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3091795"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["107(7)"],"pubmed_abstract":["<h4>Background and aims</h4>In yeasts and animals, cyclin-dependent kinases are key regulators of cell cycle progression and are negatively and positively regulated by WEE1 kinase and CDC25 phosphatase, respectively. In higher plants a full-length orthologue of CDC25 has not been isolated but a shorter gene with homology only to the C-terminal catalytic domain is present. The Arabidopis thaliana;CDC25 can act as a phosphatase in vitro. Since in arabidopsis, WEE1 plays an important role in the DNA damage/DNA replication checkpoints, the role of Arath;CDC25 in conditions that induce these checkpoints or induce abiotic stress was tested. Methods arath;cdc25 T-DNA insertion lines, Arath;CDC25 over-expressing lines and wild type were challenged with hydroxyurea (HU) and zeocin, substances that "],"journal":["Annals of botany"],"pubmed_title":["Arabidopsis T-DNA insertional lines for CDC25 are hypersensitive to hydroxyurea but not to zeocin or salt stress."],"pmcid":["PMC3091795"],"funding_grant_id":["BBS/E/J/00000129"],"pubmed_authors":["Bitonti MB","Rogers HJ","Doonan JH","Francis D","Herbert RJ","Wallace E","Spadafora ND"],"additional_accession":[]},"is_claimable":false,"name":"Arabidopsis T-DNA insertional lines for CDC25 are hypersensitive to hydroxyurea but not to zeocin or salt stress.","description":"<h4>Background and aims</h4>In yeasts and animals, cyclin-dependent kinases are key regulators of cell cycle progression and are negatively and positively regulated by WEE1 kinase and CDC25 phosphatase, respectively. In higher plants a full-length orthologue of CDC25 has not been isolated but a shorter gene with homology only to the C-terminal catalytic domain is present. The Arabidopis thaliana;CDC25 can act as a phosphatase in vitro. Since in arabidopsis, WEE1 plays an important role in the DNA damage/DNA replication checkpoints, the role of Arath;CDC25 in conditions that induce these checkpoints or induce abiotic stress was tested. Methods arath;cdc25 T-DNA insertion lines, Arath;CDC25 over-expressing lines and wild type were challenged with hydroxyurea (HU) and zeocin, substances that ","dates":{"release":"2011-01-01T00:00:00Z","publication":"2011 May","modification":"2025-04-21T21:18:02.032Z","creation":"2019-03-27T00:41:29Z"},"accession":"S-EPMC3091795","cross_references":{"pubmed":["20647223"],"doi":["10.1093/aob/mcq142"]}}