biostudies-literaturePadinhateeri RNCI NIH HHS7799-803https://www.ebi.ac.uk/biostudies/studies/S-EPMC3093463biostudies-literatureUnknown108(19)Accounting for enzyme-mediated active sliding, disassembly, and sequence-dependent positioning of nucleosomes, we simulate nucleosome occupancy over cell-cycle-scale times using a stochastic kinetic model. We show that ATP-dependent active nucleosome sliding and nucleosome removal processes are essential to obtain in vivo-like nucleosome positioning. While active sliding leads to dense nucleosome filling, sliding events alone cannot ensure sequence-dependent nucleosome positioning: Active nucleosome removal is the crucial remodeling event that drives positioning. We also show that remodeling activity changes nucleosome dynamics from glassy to liquid-like, and that remodeling dramatically influences exposure dynamics of promoter regions.Proceedings of the National Academy of Sciences of the United States of AmericaNucleosome positioning in a model of active chromatin remodeling enzymes.PMC3093463U54 CA143869U54-CA143869-01Padinhateeri RMarko JFfalseNucleosome positioning in a model of active chromatin remodeling enzymes.Accounting for enzyme-mediated active sliding, disassembly, and sequence-dependent positioning of nucleosomes, we simulate nucleosome occupancy over cell-cycle-scale times using a stochastic kinetic model. We show that ATP-dependent active nucleosome sliding and nucleosome removal processes are essential to obtain in vivo-like nucleosome positioning. While active sliding leads to dense nucleosome filling, sliding events alone cannot ensure sequence-dependent nucleosome positioning: Active nucleosome removal is the crucial remodeling event that drives positioning. We also show that remodeling activity changes nucleosome dynamics from glassy to liquid-like, and that remodeling dramatically influences exposure dynamics of promoter regions.2011-01-01T00:00:00Z2011 May2025-04-04T07:35:48.439Z2019-03-27T00:41:33ZS-EPMC30934632151890010.1073/pnas.1015206108