biostudies-literatureUnknown6(5)Shatnyeva OMProtein modifications of death receptor pathways play a central role in the regulation of apoptosis. It has been demonstrated that O-glycosylation of TRAIL-receptor (R) is essential for sensitivity and resistance towards TRAIL-mediated apoptosis. In this study we ask whether and how glycosylation of CD95 (Fas/APO-1), another death receptor, influences DISC formation and procaspase-8 activation at the CD95 DISC and thereby the onset of apoptosis. We concentrated on N-glycostructure since O-glycosylation of CD95 was not found. We applied different approaches to analyze the role of CD95 N-glycosylation on the signal transduction: in silico modeling of CD95 DISC, generation of CD95 glycosylation mutants (at N136 and N118), modulation of N-glycosylation by deoxymannojirimycin (DMM) and sialidasPloS onee19927https://www.ebi.ac.uk/biostudies/studies/S-EPMC3097226biostudies-literatureModulation of the CD95-induced apoptosis: the role of CD95 N-glycosylation.PMC3097226Schwartz-Albiez RLavrik INPappa AWeber ANWeber CEKrammer PHKubarenko AVShatnyeva OMfalseModulation of the CD95-induced apoptosis: the role of CD95 N-glycosylation.Protein modifications of death receptor pathways play a central role in the regulation of apoptosis. It has been demonstrated that O-glycosylation of TRAIL-receptor (R) is essential for sensitivity and resistance towards TRAIL-mediated apoptosis. In this study we ask whether and how glycosylation of CD95 (Fas/APO-1), another death receptor, influences DISC formation and procaspase-8 activation at the CD95 DISC and thereby the onset of apoptosis. We concentrated on N-glycostructure since O-glycosylation of CD95 was not found. We applied different approaches to analyze the role of CD95 N-glycosylation on the signal transduction: in silico modeling of CD95 DISC, generation of CD95 glycosylation mutants (at N136 and N118), modulation of N-glycosylation by deoxymannojirimycin (DMM) and sialidas2011-01-01T00:00:00Z20112026-04-07T14:27:33.382Z2019-03-26T23:13:27ZS-EPMC30972262162564410.1371/journal.pone.0019927