biostudies-literatureLister ACancer Research UKMedical Research CouncilNational Institute for Health Research (NIHR)37https://www.ebi.ac.uk/biostudies/studies/S-EPMC3098205biostudies-literatureUnknown10<h4>Background</h4>Nrf2 is a key transcriptional regulator of a battery of genes that facilitate phase II/III drug metabolism and defence against oxidative stress. Nrf2 is largely regulated by Keap1, which directs Nrf2 for proteasomal degradation. The Nrf2/Keap1 system is dysregulated in lung, head and neck, and breast cancers and this affects cellular proliferation and response to therapy. Here, we have investigated the integrity of the Nrf2/Keap1 system in pancreatic cancer.<h4>Results</h4>Keap1, Nrf2 and the Nrf2 target genes AKR1c1 and GCLC were detected in a panel of five pancreatic cancer cell lines. Mutation analysis of NRF2 exon 2 and KEAP1 exons 2-6 in these cell lines identified no mutations in NRF2 and only synonomous mutations in KEAP1. RNAi depletion of Nrf2 caused a decrease Molecular cancerNrf2 is overexpressed in pancreatic cancer: implications for cell proliferation and therapy.PMC3098205G0700654NF-SI-0510-10126C6665/A7348Costello ENeoptolemos JPKitteringham NRLloyd BCampbell FGoldring CEOwen ANedjadi TLister ACopple IMWilliams SPark BKfalseNrf2 is overexpressed in pancreatic cancer: implications for cell proliferation and therapy.<h4>Background</h4>Nrf2 is a key transcriptional regulator of a battery of genes that facilitate phase II/III drug metabolism and defence against oxidative stress. Nrf2 is largely regulated by Keap1, which directs Nrf2 for proteasomal degradation. The Nrf2/Keap1 system is dysregulated in lung, head and neck, and breast cancers and this affects cellular proliferation and response to therapy. Here, we have investigated the integrity of the Nrf2/Keap1 system in pancreatic cancer.<h4>Results</h4>Keap1, Nrf2 and the Nrf2 target genes AKR1c1 and GCLC were detected in a panel of five pancreatic cancer cell lines. Mutation analysis of NRF2 exon 2 and KEAP1 exons 2-6 in these cell lines identified no mutations in NRF2 and only synonomous mutations in KEAP1. RNAi depletion of Nrf2 caused a decrease 2011-01-01T00:00:00Z2011 Apr2026-04-07T15:15:57.011Z2019-03-27T00:41:47ZS-EPMC30982052148925710.1186/1476-4598-10-37