{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["6(5)"],"submitter":["Zvi A"],"pubmed_abstract":["The cellular arm of the immune response plays a central role in the defense against intracellular pathogens, such as F. tularensis. To date, whole genome immunoinformatic analyses were limited either to relatively small genomes (e.g. viral) or to preselected subsets of proteins in complex pathogens. Here we present, for the first time, an unbiased bacterial global immunoinformatic screen of the 1740 proteins of F. tularensis subs. holarctica (LVS), aiming at identification of immunogenic peptides eliciting a CTL response. The very large number of predicted MHC class I binders (about 100,000, IC(50) of 1000 nM or less) required the design of a strategy for further down selection of CTL candidates. The approach developed focused on mapping clusters rich in overlapping predicted epitopes, and"],"journal":["PloS one"],"pagination":["e20050"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3098878"],"repository":["biostudies-literature"],"pubmed_title":["Whole-genome immunoinformatic analysis of F. tularensis: predicted CTL epitopes clustered in hotspots are prone to elicit a T-cell response."],"pmcid":["PMC3098878"],"pubmed_authors":["Cohen O","Zvi A","Rotem S","Bar-Haim E","Shafferman A"],"additional_accession":[]},"is_claimable":false,"name":"Whole-genome immunoinformatic analysis of F. tularensis: predicted CTL epitopes clustered in hotspots are prone to elicit a T-cell response.","description":"The cellular arm of the immune response plays a central role in the defense against intracellular pathogens, such as F. tularensis. To date, whole genome immunoinformatic analyses were limited either to relatively small genomes (e.g. viral) or to preselected subsets of proteins in complex pathogens. Here we present, for the first time, an unbiased bacterial global immunoinformatic screen of the 1740 proteins of F. tularensis subs. holarctica (LVS), aiming at identification of immunogenic peptides eliciting a CTL response. The very large number of predicted MHC class I binders (about 100,000, IC(50) of 1000 nM or less) required the design of a strategy for further down selection of CTL candidates. The approach developed focused on mapping clusters rich in overlapping predicted epitopes, and","dates":{"release":"2011-01-01T00:00:00Z","publication":"2011","modification":"2026-04-18T11:03:27.802Z","creation":"2019-03-26T23:13:27Z"},"accession":"S-EPMC3098878","cross_references":{"pubmed":["21625462"],"doi":["10.1371/journal.pone.0020050"]}}