{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Keita D"],"funding":["European Commission FP7","Wellcome Trust"],"pagination":["727-36"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3101891"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(5)"],"pubmed_abstract":["<h4>Background</h4>African swine fever virus (ASFV) is the unique member of the Asfarviridae family and Asfivirus genus. It is an enveloped double-stranded DNA arbovirus that replicates in the cell cytoplasm, similar to poxviruses. There is no vaccine and no treatment available to control this virus.<h4>Methods</h4>We describe the use of small interfering RNA (siRNA) targeting the A151R and VP72 (B646L) genes to control the ASFV replication in vitro.<h4>Results</h4>Results suggest that siRNA targeting the A151R and VP72 genes can reduce both the virus replication and its levels of messenger RNA transcripts. The reduction was up to 4 log(10) copies on the virus titre and up to 3 log(10) copies on virus RNA transcripts levels. The combination of multiple siRNA did not improve the antiviral e"],"journal":["Antiviral therapy"],"pubmed_title":["Control of African swine fever virus replication by small interfering RNA targeting the A151R and VP72 genes."],"pmcid":["PMC3101891"],"funding_grant_id":["075813","AHDW/03/04","FP7_211691"],"pubmed_authors":["Heath L","Albina E","Keita D"],"additional_accession":[]},"is_claimable":false,"name":"Control of African swine fever virus replication by small interfering RNA targeting the A151R and VP72 genes.","description":"<h4>Background</h4>African swine fever virus (ASFV) is the unique member of the Asfarviridae family and Asfivirus genus. It is an enveloped double-stranded DNA arbovirus that replicates in the cell cytoplasm, similar to poxviruses. There is no vaccine and no treatment available to control this virus.<h4>Methods</h4>We describe the use of small interfering RNA (siRNA) targeting the A151R and VP72 (B646L) genes to control the ASFV replication in vitro.<h4>Results</h4>Results suggest that siRNA targeting the A151R and VP72 genes can reduce both the virus replication and its levels of messenger RNA transcripts. The reduction was up to 4 log(10) copies on the virus titre and up to 3 log(10) copies on virus RNA transcripts levels. The combination of multiple siRNA did not improve the antiviral e","dates":{"release":"2010-01-01T00:00:00Z","publication":"2010","modification":"2025-04-26T04:26:06.338Z","creation":"2019-06-05T16:29:44Z"},"accession":"S-EPMC3101891","cross_references":{"pubmed":["20710054"],"doi":["10.3851/IMP1593","10.3851/imp1593"]}}