biostudies-literatureBoucrot ENIGMS NIH HHS1965-72https://www.ebi.ac.uk/biostudies/studies/S-EPMC3104031biostudies-literatureUnknown124(Pt 12)Caveolae form a specialized platform within the plasma membrane that is crucial for an array of important biological functions, ranging from signaling to endocytosis. Using total internal reflection fluorescence (TIRF) and 3D fast spinning-disk confocal imaging to follow caveola dynamics for extended periods, and electron microscopy to obtain high resolution snapshots, we found that the vast majority of caveolae are dynamic with lifetimes ranging from a few seconds to several minutes. Use of these methods revealed a change in the dynamics and localization of caveolae during mitosis. During interphase, the equilibrium between the arrival and departure of caveolae from the cell surface maintains the steady-state distribution of caveolin-1 (Cav1) at the plasma membrane. During mitosis, increaJournal of cell scienceRedistribution of caveolae during mitosis.PMC3104031P01 GM062566GM 62566R01 GM075252GM 075252Kirchhausen THowes MTParton RGBoucrot EfalseRedistribution of caveolae during mitosis.Caveolae form a specialized platform within the plasma membrane that is crucial for an array of important biological functions, ranging from signaling to endocytosis. Using total internal reflection fluorescence (TIRF) and 3D fast spinning-disk confocal imaging to follow caveola dynamics for extended periods, and electron microscopy to obtain high resolution snapshots, we found that the vast majority of caveolae are dynamic with lifetimes ranging from a few seconds to several minutes. Use of these methods revealed a change in the dynamics and localization of caveolae during mitosis. During interphase, the equilibrium between the arrival and departure of caveolae from the cell surface maintains the steady-state distribution of caveolin-1 (Cav1) at the plasma membrane. During mitosis, increa2011-01-01T00:00:00Z2011 Jun2025-04-22T08:48:01.916Z2019-03-27T00:42:06ZS-EPMC31040312162500710.1242/jcs.076570