{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Koo AJ"],"funding":["NIGMS NIH HHS"],"pagination":["9298-303"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3107288"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["108(22)"],"pubmed_abstract":["The phytohormone jasmonoyl-L-isoleucine (JA-Ile) signals through the COI1-JAZ coreceptor complex to control key aspects of plant growth, development, and immune function. Despite detailed knowledge of the JA-Ile biosynthetic pathway, little is known about the genetic basis of JA-Ile catabolism and inactivation. Here, we report the identification of a wound- and jasmonate-responsive gene from Arabidopsis that encodes a cytochrome P450 (CYP94B3) involved in JA-Ile turnover. Metabolite analysis of wounded leaves showed that loss of CYP94B3 function in cyp94b3 mutants causes hyperaccumulation of JA-Ile and concomitant reduction in 12-hydroxy-JA-Ile (12OH-JA-Ile) content, whereas overexpression of this enzyme results in severe depletion of JA-Ile and corresponding changes in 12OH-JA-Ile levels."],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Cytochrome P450 CYP94B3 mediates catabolism and inactivation of the plant hormone jasmonoyl-L-isoleucine."],"pmcid":["PMC3107288"],"funding_grant_id":["R01GM57795","R01 GM057795"],"pubmed_authors":["Howe GA","Koo AJ","Cooke TF"],"additional_accession":[]},"is_claimable":false,"name":"Cytochrome P450 CYP94B3 mediates catabolism and inactivation of the plant hormone jasmonoyl-L-isoleucine.","description":"The phytohormone jasmonoyl-L-isoleucine (JA-Ile) signals through the COI1-JAZ coreceptor complex to control key aspects of plant growth, development, and immune function. Despite detailed knowledge of the JA-Ile biosynthetic pathway, little is known about the genetic basis of JA-Ile catabolism and inactivation. Here, we report the identification of a wound- and jasmonate-responsive gene from Arabidopsis that encodes a cytochrome P450 (CYP94B3) involved in JA-Ile turnover. Metabolite analysis of wounded leaves showed that loss of CYP94B3 function in cyp94b3 mutants causes hyperaccumulation of JA-Ile and concomitant reduction in 12-hydroxy-JA-Ile (12OH-JA-Ile) content, whereas overexpression of this enzyme results in severe depletion of JA-Ile and corresponding changes in 12OH-JA-Ile levels.","dates":{"release":"2011-01-01T00:00:00Z","publication":"2011 May","modification":"2026-07-15T13:40:41.395Z","creation":"2025-07-08T03:12:48.465Z"},"accession":"S-EPMC3107288","cross_references":{"pubmed":["21576464"],"doi":["10.1073/pnas.1103542108"]}}