{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kiraly O"],"funding":["NIDDK NIH HHS"],"pagination":["175-94"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3107599"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["705"],"pubmed_abstract":["Heterologously expressed proteins in Escherichia coli may undergo unwanted N-terminal processing by methionine and proline aminopeptidases. To overcome this problem, we present a system where the gene of interest is cloned as a fusion to a self-splicing mini-intein. This fusion construct is expressed in an engineered E. coli strain from which the pepP gene coding for aminopeptidase P has been deleted. We describe a protocol using human cationic trypsinogen as an example to demonstrate that recombinant proteins produced in this expression system contain homogeneous, unprocessed N-termini."],"journal":["Methods in molecular biology (Clifton, N.J.)"],"pubmed_title":["Expression of recombinant proteins with uniform N-termini."],"pmcid":["PMC3107599"],"funding_grant_id":["R01 DK058088-06","R01 DK058088-07","R01 DK058088","DK058088","R01 DK058088-05A1"],"pubmed_authors":["Kiraly O","Guan L","Sahin-Toth M"],"additional_accession":[]},"is_claimable":false,"name":"Expression of recombinant proteins with uniform N-termini.","description":"Heterologously expressed proteins in Escherichia coli may undergo unwanted N-terminal processing by methionine and proline aminopeptidases. To overcome this problem, we present a system where the gene of interest is cloned as a fusion to a self-splicing mini-intein. This fusion construct is expressed in an engineered E. coli strain from which the pepP gene coding for aminopeptidase P has been deleted. We describe a protocol using human cationic trypsinogen as an example to demonstrate that recombinant proteins produced in this expression system contain homogeneous, unprocessed N-termini.","dates":{"release":"2011-01-01T00:00:00Z","publication":"2011","modification":"2025-04-27T00:40:59.033Z","creation":"2019-06-05T16:30:49Z"},"accession":"S-EPMC3107599","cross_references":{"pubmed":["21125386"],"doi":["10.1007/978-1-61737-967-3_10"]}}