biostudies-literatureSerre KMedical Research CouncilWellcome TrustBiotechnology and Biological Sciences Research Councile20731https://www.ebi.ac.uk/biostudies/studies/S-EPMC3108993biostudies-literatureUnknown6(6)<h4>Background</h4>Although in vitro IL-4 directs CD4 T cells to produce T helper 2 (Th2)-cytokines, these cytokines can be induced in vivo in the absence of IL-4-signalling. Thus, mechanism(s), different from the in vitro pathway for Th2-induction, contribute to in vivo Th2-differentiation. The pathway for in vivo IL-4-independent Th2-differentiation has yet to be characterized.<h4>Findings</h4>Helios (ikzf2), a member of the Ikaros transcription regulator family, is expressed in thymocytes and some antigen-matured T cells as well as in regulatory T cells. It has been proposed that Helios is a specific marker for thymus-derived regulatory T cells. Here, we show that mouse ovalbumin-specific CD4 (OTII) cells responding to alum-precipitated ovalbumin (alumOVA) upregulate Th2 features - GATAPloS oneHelios is associated with CD4 T cells differentiating to T helper 2 and follicular helper T cells in vivo independently of Foxp3 expression.PMC3108993G8402371G9818340G9818340BG0701275BB/F022778/1Desanti GBobat SMohr ESerre KCunningham AFBird RToellner KMMaclennan ICChan SKastner PBenezech CfalseHelios is associated with CD4 T cells differentiating to T helper 2 and follicular helper T cells in vivo independently of Foxp3 expression.<h4>Background</h4>Although in vitro IL-4 directs CD4 T cells to produce T helper 2 (Th2)-cytokines, these cytokines can be induced in vivo in the absence of IL-4-signalling. Thus, mechanism(s), different from the in vitro pathway for Th2-induction, contribute to in vivo Th2-differentiation. The pathway for in vivo IL-4-independent Th2-differentiation has yet to be characterized.<h4>Findings</h4>Helios (ikzf2), a member of the Ikaros transcription regulator family, is expressed in thymocytes and some antigen-matured T cells as well as in regulatory T cells. It has been proposed that Helios is a specific marker for thymus-derived regulatory T cells. Here, we show that mouse ovalbumin-specific CD4 (OTII) cells responding to alum-precipitated ovalbumin (alumOVA) upregulate Th2 features - GATA2011-01-01T00:00:00Z20112026-04-14T13:36:50.811Z2025-05-18T11:23:32.848ZS-EPMC31089932167777810.1371/journal.pone.0020731