biostudies-literatureUnknown13(1)Bourdon JCCancer Research UK<h4>Introduction</h4>Normal function of the p53 network is lost in most cancers, often through p53 mutation. The clinical impact of p53 mutations in breast cancer remains uncertain, especially where p53 isoforms may modify the effects of these p53 mutations.<h4>Methods</h4>Expression of p53? and p53? isoforms, the isoforms identified in normal breast tissue, was detected by reverse transcription polymerase chain reaction from a cohort of 127 primary breast tumours. Expression of p53? and p53? isoforms was analysed in relation to clinical markers and clinical outcomes (5 years) by binary logistic regression, Cox proportional hazards regression and Kaplan-Meier survival analyses.<h4>Results</h4>p53? and p53? were not randomly expressed in breast cancer. p53? was associated with tumour oestrogen receptor (ER) expression, and p53? was associated with mutation of the p53 gene. The patient group with the mutant p53 breast tumour-expressing p53? isoform had low cancer recurrence and an overall survival as good as that of patients with wild-type p53 breast cancer. Conversely, patients expressing only mutant p53, without p53? isoform expression, had a particularly poor prognosis.<h4>Conclusions</h4>The determination of p53? expression may allow the identification, independently of the ER status, of two subpopulations of mutant p53 breast cancer patients, one expressing p53? with a prognosis as good as the wild-type p53 breast cancer patients and a second one not expressing p53? with a particularly poor prognosis. The p53? isoform may provide an explanation of the hitherto inconsistent relationship between p53 mutation, treatment response and outcome in breast cancer.Breast cancer research : BCRR7https://www.ebi.ac.uk/biostudies/studies/S-EPMC3109573biostudies-literaturep53 mutant breast cancer patients expressing p53? have as good a prognosis as wild-type p53 breast cancer patients.PMC3109573Baker LQuinlan PPrats ACKhoury MPPurdie CAAoubala MFernandes KThompson AMJordan LBLane DPBourdon JCDiot Afalsep53 mutant breast cancer patients expressing p53? have as good a prognosis as wild-type p53 breast cancer patients.<h4>Introduction</h4>Normal function of the p53 network is lost in most cancers, often through p53 mutation. The clinical impact of p53 mutations in breast cancer remains uncertain, especially where p53 isoforms may modify the effects of these p53 mutations.<h4>Methods</h4>Expression of p53? and p53? isoforms, the isoforms identified in normal breast tissue, was detected by reverse transcription polymerase chain reaction from a cohort of 127 primary breast tumours. Expression of p53? and p53? isoforms was analysed in relation to clinical markers and clinical outcomes (5 years) by binary logistic regression, Cox proportional hazards regression and Kaplan-Meier survival analyses.<h4>Results</h4>p53? and p53? were not randomly expressed in breast cancer. p53? was associated with tumour oestrogen receptor (ER) expression, and p53? was associated with mutation of the p53 gene. The patient group with the mutant p53 breast tumour-expressing p53? isoform had low cancer recurrence and an overall survival as good as that of patients with wild-type p53 breast cancer. Conversely, patients expressing only mutant p53, without p53? isoform expression, had a particularly poor prognosis.<h4>Conclusions</h4>The determination of p53? expression may allow the identification, independently of the ER status, of two subpopulations of mutant p53 breast cancer patients, one expressing p53? with a prognosis as good as the wild-type p53 breast cancer patients and a second one not expressing p53? with a particularly poor prognosis. The p53? isoform may provide an explanation of the hitherto inconsistent relationship between p53 mutation, treatment response and outcome in breast cancer.2011-01-01T00:00:00Z2011 Jan2021-02-25T08:37:38Z2019-03-27T00:42:20ZS-EPMC31095732125132910.1186/bcr2811