{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Brumovsky PR"],"funding":["NINDS NIH HHS"],"pagination":["258-72"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3114170"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["230(2)"],"pubmed_abstract":["Glutamate is the main excitatory neurotransmitter in the nervous system, including in primary afferent neurons. However, to date a glutamatergic phenotype of autonomic neurons has not been described. Therefore, we explored the expression of vesicular glutamate transporter (VGLUT) types 1, 2 and 3 in lumbar sympathetic chain (LSC) and major pelvic ganglion (MPG) of naïve BALB/C mice, as well as after pelvic nerve axotomy (PNA), using immunohistochemistry and in situ hybridization. Colocalization with activating transcription factor-3 (ATF-3), tyrosine hydroxylase (TH), vesicular acetylcholine transporter (VAChT) and calcitonin gene-related peptide was also examined. Sham-PNA, sciatic nerve axotomy (SNA) or naïve mice were included. In naïve mice, VGLUT(2)-like immunoreactivity (LI) was only"],"journal":["Experimental neurology"],"pubmed_title":["Some lumbar sympathetic neurons develop a glutamatergic phenotype after peripheral axotomy with a note on VGLUT₂-positive perineuronal baskets."],"pmcid":["PMC3114170"],"funding_grant_id":["NS 35790","R01 NS035790-14","R01 NS035790","NS19912","R01 NS019912-28","R01 NS019912"],"pubmed_authors":["Brumovsky PR","Seroogy KB","Hokfelt T","Lundgren KH","Watanabe M","Gebhart GF"],"additional_accession":[]},"is_claimable":false,"name":"Some lumbar sympathetic neurons develop a glutamatergic phenotype after peripheral axotomy with a note on VGLUT₂-positive perineuronal baskets.","description":"Glutamate is the main excitatory neurotransmitter in the nervous system, including in primary afferent neurons. However, to date a glutamatergic phenotype of autonomic neurons has not been described. Therefore, we explored the expression of vesicular glutamate transporter (VGLUT) types 1, 2 and 3 in lumbar sympathetic chain (LSC) and major pelvic ganglion (MPG) of naïve BALB/C mice, as well as after pelvic nerve axotomy (PNA), using immunohistochemistry and in situ hybridization. Colocalization with activating transcription factor-3 (ATF-3), tyrosine hydroxylase (TH), vesicular acetylcholine transporter (VAChT) and calcitonin gene-related peptide was also examined. Sham-PNA, sciatic nerve axotomy (SNA) or naïve mice were included. In naïve mice, VGLUT(2)-like immunoreactivity (LI) was only","dates":{"release":"2011-01-01T00:00:00Z","publication":"2011 Aug","modification":"2025-06-01T01:07:44.298Z","creation":"2019-06-05T16:32:15Z"},"accession":"S-EPMC3114170","cross_references":{"pubmed":["21596036"],"doi":["10.1016/j.expneurol.2011.05.004"]}}