{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yang H"],"funding":["NCCIH NIH HHS","NIDDK NIH HHS"],"pagination":["378-88, 388.e1-4"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3129489"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["141(1)"],"pubmed_abstract":["<h4>Background & aims</h4>Cholestasis contributes to hepatocellular injury and promotes liver carcinogenesis. We created a mouse model of chronic cholestasis to study its effects on progression of cholangiocarcinoma and the oncogenes involved.<h4>Methods</h4>To induce chronic cholestasis, Balb/c mice were given 2 weekly intraperitoneal injections of diethylnitrosamine (DEN); 2 weeks later, some mice also received left and median bile duct ligation (LMBDL) and, then 1 week later, were fed DEN, in corn oil, weekly by oral gavage (DLD). Liver samples were analyzed by immunohistochemical and biochemical assays; expression of Mnt and c-Myc was reduced by injection of small inhibitor RNAs.<h4>Results</h4>Chronic cholestasis was induced by DLD and accelerated progression of cholangiocarcinoma, co"],"journal":["Gastroenterology"],"pubmed_title":["A mouse model of cholestasis-associated cholangiocarcinoma and transcription factors involved in progression."],"pmcid":["PMC3129489"],"funding_grant_id":["R01 AT001576","R01 DK045334-15","R01 DK045334","P30 DK048522","R01 AT001576-09","DK45334","R01 DK051719","P30DK48522","AT1576","P30 DK048522-15","DK51719","R56 DK045334","R01 DK051719-14"],"pubmed_authors":["Yang H","Xia M","Ko KS","Li TW","Aller MA","Peng J","Tang X"],"additional_accession":[]},"is_claimable":false,"name":"A mouse model of cholestasis-associated cholangiocarcinoma and transcription factors involved in progression.","description":"<h4>Background & aims</h4>Cholestasis contributes to hepatocellular injury and promotes liver carcinogenesis. We created a mouse model of chronic cholestasis to study its effects on progression of cholangiocarcinoma and the oncogenes involved.<h4>Methods</h4>To induce chronic cholestasis, Balb/c mice were given 2 weekly intraperitoneal injections of diethylnitrosamine (DEN); 2 weeks later, some mice also received left and median bile duct ligation (LMBDL) and, then 1 week later, were fed DEN, in corn oil, weekly by oral gavage (DLD). Liver samples were analyzed by immunohistochemical and biochemical assays; expression of Mnt and c-Myc was reduced by injection of small inhibitor RNAs.<h4>Results</h4>Chronic cholestasis was induced by DLD and accelerated progression of cholangiocarcinoma, co","dates":{"release":"2011-01-01T00:00:00Z","publication":"2011 Jul","modification":"2025-04-21T20:42:39.042Z","creation":"2019-03-27T03:06:40Z"},"accession":"S-EPMC3129489","cross_references":{"pubmed":["21440549"],"doi":["10.1053/j.gastro.2011.03.044"]}}