biostudies-literatureYang HNCCIH NIH HHSNIDDK NIH HHS378-88, 388.e1-4https://www.ebi.ac.uk/biostudies/studies/S-EPMC3129489biostudies-literatureUnknown141(1)<h4>Background & aims</h4>Cholestasis contributes to hepatocellular injury and promotes liver carcinogenesis. We created a mouse model of chronic cholestasis to study its effects on progression of cholangiocarcinoma and the oncogenes involved.<h4>Methods</h4>To induce chronic cholestasis, Balb/c mice were given 2 weekly intraperitoneal injections of diethylnitrosamine (DEN); 2 weeks later, some mice also received left and median bile duct ligation (LMBDL) and, then 1 week later, were fed DEN, in corn oil, weekly by oral gavage (DLD). Liver samples were analyzed by immunohistochemical and biochemical assays; expression of Mnt and c-Myc was reduced by injection of small inhibitor RNAs.<h4>Results</h4>Chronic cholestasis was induced by DLD and accelerated progression of cholangiocarcinoma, coGastroenterologyA mouse model of cholestasis-associated cholangiocarcinoma and transcription factors involved in progression.PMC3129489R01 AT001576R01 DK045334-15R01 DK045334P30 DK048522R01 AT001576-09DK45334R01 DK051719P30DK48522AT1576P30 DK048522-15DK51719R56 DK045334R01 DK051719-14Yang HXia MKo KSLi TWAller MAPeng JTang XfalseA mouse model of cholestasis-associated cholangiocarcinoma and transcription factors involved in progression.<h4>Background & aims</h4>Cholestasis contributes to hepatocellular injury and promotes liver carcinogenesis. We created a mouse model of chronic cholestasis to study its effects on progression of cholangiocarcinoma and the oncogenes involved.<h4>Methods</h4>To induce chronic cholestasis, Balb/c mice were given 2 weekly intraperitoneal injections of diethylnitrosamine (DEN); 2 weeks later, some mice also received left and median bile duct ligation (LMBDL) and, then 1 week later, were fed DEN, in corn oil, weekly by oral gavage (DLD). Liver samples were analyzed by immunohistochemical and biochemical assays; expression of Mnt and c-Myc was reduced by injection of small inhibitor RNAs.<h4>Results</h4>Chronic cholestasis was induced by DLD and accelerated progression of cholangiocarcinoma, co2011-01-01T00:00:00Z2011 Jul2025-04-21T20:42:39.042Z2019-03-27T03:06:40ZS-EPMC31294892144054910.1053/j.gastro.2011.03.044