{"database":"biostudies-literature","file_versions":[],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":59,"searchCount":0},"additional":{"omics_type":["Unknown"],"volume":["18(6)"],"submitter":["Afanasyeva EA"],"pubmed_abstract":["Several microRNA (miRNA) loci are found within genomic regions frequently deleted in primary neuroblastoma, including miR-885-5p at 3p25.3. In this study, we demonstrate that miR-885-5p is downregulated on loss of 3p25.3 region in neuroblastoma. Experimentally enforced miR-885-5p expression in neuroblastoma cell lines inhibits proliferation triggering cell cycle arrest, senescence and/or apoptosis. miR-885-5p leads to the accumulation of p53 protein and activates the p53 pathway, resulting in upregulation of p53 targets. Enforced miR-885-5p expression consistently leads to downregulation of cyclin-dependent kinase (CDK2) and mini-chromosome maintenance protein (MCM5). Both genes are targeted by miR-885-5p via predicted binding sites within the 3'-untranslated regions (UTRs) of CDK2 and MCM5. Transcript profiling after miR-885-5p introduction in neuroblastoma cells reveals alterations in expression of multiple genes, including several p53 target genes and a number of factors involved in p53 pathway activity. Taken together, these data provide evidence that miR-885-5p has a tumor suppressive role in neuroblastoma interfering with cell cycle progression and cell survival."],"journal":["Cell death and differentiation"],"pagination":["974-84"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3131937"],"repository":["biostudies-literature"],"pubmed_title":["MicroRNA miR-885-5p targets CDK2 and MCM5, activates p53 and inhibits proliferation and survival."],"pmcid":["PMC3131937"],"pubmed_authors":["Ehemann V","Speleman F","Kumps C","Theissen J","Zapatka M","Afanasyeva EA","Savelyeva L","Schwab M","Sagulenko V","Vandesompele J","Brors B","Westermann F","Fischer M","Mestdagh P"],"view_count":["59"],"additional_accession":[]},"is_claimable":false,"name":"MicroRNA miR-885-5p targets CDK2 and MCM5, activates p53 and inhibits proliferation and survival.","description":"Several microRNA (miRNA) loci are found within genomic regions frequently deleted in primary neuroblastoma, including miR-885-5p at 3p25.3. In this study, we demonstrate that miR-885-5p is downregulated on loss of 3p25.3 region in neuroblastoma. Experimentally enforced miR-885-5p expression in neuroblastoma cell lines inhibits proliferation triggering cell cycle arrest, senescence and/or apoptosis. miR-885-5p leads to the accumulation of p53 protein and activates the p53 pathway, resulting in upregulation of p53 targets. Enforced miR-885-5p expression consistently leads to downregulation of cyclin-dependent kinase (CDK2) and mini-chromosome maintenance protein (MCM5). Both genes are targeted by miR-885-5p via predicted binding sites within the 3'-untranslated regions (UTRs) of CDK2 and MCM5. Transcript profiling after miR-885-5p introduction in neuroblastoma cells reveals alterations in expression of multiple genes, including several p53 target genes and a number of factors involved in p53 pathway activity. Taken together, these data provide evidence that miR-885-5p has a tumor suppressive role in neuroblastoma interfering with cell cycle progression and cell survival.","dates":{"release":"2011-01-01T00:00:00Z","publication":"2011 Jun","modification":"2024-11-21T05:00:59.866Z","creation":"2019-03-27T03:06:53Z"},"accession":"S-EPMC3131937","cross_references":{"pubmed":["21233845"],"doi":["10.1038/cdd.2010.164"]}}