biostudies-literatureUnknown4Conzelmann N<h4>Background</h4>Granulocyte-colony stimulating factor (G-CSF) is one of the most important pharmacologically used proteins. Potential uses beyond the stimulation of neutrophilic granulocytes are the treatment of CNS disorders. Disadvantages of the G-CSF protein as a drug are its moderate plasma half-life time and considerable production costs. We therefore conducted a screen for peptide agonists derived from the sequence of human G-CSF.<h4>Findings</h4>Despite of the high sensitivity of our screening system we could not detect any positive hits in a single peptide approach. In a multiplex approach using a permutation of any combination of 10 different peptides we could also not detect a positive block.<h4>Conclusions</h4>We conclude that larger coherent parts of the protein or dimerisinBMC research notes194https://www.ebi.ac.uk/biostudies/studies/S-EPMC3132715biostudies-literatureA screen for peptide agonists of the G-CSF receptor.PMC3132715Conzelmann NSchneider AfalseA screen for peptide agonists of the G-CSF receptor.<h4>Background</h4>Granulocyte-colony stimulating factor (G-CSF) is one of the most important pharmacologically used proteins. Potential uses beyond the stimulation of neutrophilic granulocytes are the treatment of CNS disorders. Disadvantages of the G-CSF protein as a drug are its moderate plasma half-life time and considerable production costs. We therefore conducted a screen for peptide agonists derived from the sequence of human G-CSF.<h4>Findings</h4>Despite of the high sensitivity of our screening system we could not detect any positive hits in a single peptide approach. In a multiplex approach using a permutation of any combination of 10 different peptides we could also not detect a positive block.<h4>Conclusions</h4>We conclude that larger coherent parts of the protein or dimerisin2011-01-01T00:00:00Z2011 Jun2025-05-29T20:30:19.394Z2019-03-27T03:06:54ZS-EPMC31327152167623910.1186/1756-0500-4-194