{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Gu C"],"funding":["NINDS NIH HHS","PHS HHS"],"pagination":["25835-47"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3138291"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["286(29)"],"pubmed_abstract":["Precise localization of axonal ion channels is crucial for proper electrical and chemical functions of axons. In myelinated axons, Kv1 (Shaker) voltage-gated potassium (Kv) channels are clustered in the juxtaparanodal regions flanking the node of Ranvier. The clustering can be disrupted by deletion of various proteins in mice, including contactin-associated protein-like 2 (Caspr2) and transient axonal glycoprotein-1 (TAG-1), a glycosylphosphatidylinositol-anchored cell adhesion molecule. However, the mechanism and function of Kv1 juxtaparanodal clustering remain unclear. Here, using a new myelin coculture of hippocampal neurons and oligodendrocytes, we report that tyrosine phosphorylation plays a critical role in TAG-1-mediated clustering of axonal Kv1.2 channels. In the coculture, myelin "],"journal":["The Journal of biological chemistry"],"pubmed_title":["Clustering and activity tuning of Kv1 channels in myelinated hippocampal axons."],"pmcid":["PMC3138291"],"funding_grant_id":["TA3012A1","R01 NS062720","R01NS062720"],"pubmed_authors":["Gu C","Gu Y"],"additional_accession":[]},"is_claimable":false,"name":"Clustering and activity tuning of Kv1 channels in myelinated hippocampal axons.","description":"Precise localization of axonal ion channels is crucial for proper electrical and chemical functions of axons. In myelinated axons, Kv1 (Shaker) voltage-gated potassium (Kv) channels are clustered in the juxtaparanodal regions flanking the node of Ranvier. The clustering can be disrupted by deletion of various proteins in mice, including contactin-associated protein-like 2 (Caspr2) and transient axonal glycoprotein-1 (TAG-1), a glycosylphosphatidylinositol-anchored cell adhesion molecule. However, the mechanism and function of Kv1 juxtaparanodal clustering remain unclear. Here, using a new myelin coculture of hippocampal neurons and oligodendrocytes, we report that tyrosine phosphorylation plays a critical role in TAG-1-mediated clustering of axonal Kv1.2 channels. In the coculture, myelin ","dates":{"release":"2011-01-01T00:00:00Z","publication":"2011 Jul","modification":"2025-04-05T10:03:08.583Z","creation":"2019-03-27T03:07:10Z"},"accession":"S-EPMC3138291","cross_references":{"pubmed":["21602278"],"doi":["10.1074/jbc.M111.219113","10.1074/jbc.m111.219113"]}}