{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["McGowan PM"],"funding":["Intramural NIH HHS","CIHR"],"pagination":["834-44"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3140630"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(7)"],"pubmed_abstract":["Brain metastasis from breast cancer is an increasingly important clinical problem. Here we assessed the role of CD44(hi)/CD24(lo) cells and pathways that regulate them, in an experimental model of brain metastasis. Notch signaling (mediated by γ-secretase) has been shown to contribute to maintenance of the cancer stem cell (CSC) phenotype. Cells sorted for a reduced stem-like phenotype had a reduced ability to form brain metastases compared with unsorted or CD44(hi)/CD24(lo) cells (P < 0.05; Kruskal-Wallis). To assess the effect of γ-secretase inhibition, cells were cultured with DAPT and the CD44/CD24 phenotypes quantified. 231-BR cells with a CD44(hi)/CD24(lo) phenotype was reduced by about 15% in cells treated with DAPT compared with DMSO-treated or untreated cells (P = 0.001, ANOVA). I"],"journal":["Molecular cancer research : MCR"],"pubmed_title":["Notch1 inhibition alters the CD44hi/CD24lo population and reduces the formation of brain metastases from breast cancer."],"pmcid":["PMC3140630"],"funding_grant_id":["ZIA BC010538"],"pubmed_authors":["Palmieri D","Steeg PS","Chambers AF","McGowan PM","Foster PJ","Simedrea C","Ribot EJ","Allan AL"],"additional_accession":[]},"is_claimable":false,"name":"Notch1 inhibition alters the CD44hi/CD24lo population and reduces the formation of brain metastases from breast cancer.","description":"Brain metastasis from breast cancer is an increasingly important clinical problem. Here we assessed the role of CD44(hi)/CD24(lo) cells and pathways that regulate them, in an experimental model of brain metastasis. Notch signaling (mediated by γ-secretase) has been shown to contribute to maintenance of the cancer stem cell (CSC) phenotype. Cells sorted for a reduced stem-like phenotype had a reduced ability to form brain metastases compared with unsorted or CD44(hi)/CD24(lo) cells (P < 0.05; Kruskal-Wallis). To assess the effect of γ-secretase inhibition, cells were cultured with DAPT and the CD44/CD24 phenotypes quantified. 231-BR cells with a CD44(hi)/CD24(lo) phenotype was reduced by about 15% in cells treated with DAPT compared with DMSO-treated or untreated cells (P = 0.001, ANOVA). I","dates":{"release":"2011-01-01T00:00:00Z","publication":"2011 Jul","modification":"2026-03-15T21:05:25.578Z","creation":"2025-08-13T03:06:24.193Z"},"accession":"S-EPMC3140630","cross_references":{"pubmed":["21665937"],"doi":["10.1158/1541-7786.MCR-10-0457","10.1158/1541-7786.mcr-10-0457"]}}