biostudies-literatureUnknown108(2)Leroux O<h4>Background and aims</h4>The anatomy of Equisetum stems is characterized by the occurrence of vallecular and carinal canals. Previous studies on the carinal canals in several Equisetum species suggest that they convey water from one node to another.<h4>Methods</h4>Cell wall composition and ultrastructure have been studied using immunocytochemistry and electron microscopy, respectively. Serial sectioning and X-ray computed tomography were employed to examine the internode-node-internode transition of Equisetum ramosissimum.<h4>Key results</h4>The distribution of the LM1 and JIM20 extensin epitopes is restricted to the lining of carinal canals. The monoclonal antibodies JIM5 and LM19 directed against homogalacturonan with a low degree of methyl esterification and the CBM3a probe recogniziAnnals of botany307-19https://www.ebi.ac.uk/biostudies/studies/S-EPMC3143055biostudies-literatureAn extensin-rich matrix lines the carinal canals in Equisetum ramosissimum, which may function as water-conducting channels.PMC3143055Viane RLKnox JPLeroux OBagniewska-Zadworna AClaeys MMarcus SEvan Hoorebeke LMasschaele BfalseAn extensin-rich matrix lines the carinal canals in Equisetum ramosissimum, which may function as water-conducting channels.<h4>Background and aims</h4>The anatomy of Equisetum stems is characterized by the occurrence of vallecular and carinal canals. Previous studies on the carinal canals in several Equisetum species suggest that they convey water from one node to another.<h4>Methods</h4>Cell wall composition and ultrastructure have been studied using immunocytochemistry and electron microscopy, respectively. Serial sectioning and X-ray computed tomography were employed to examine the internode-node-internode transition of Equisetum ramosissimum.<h4>Key results</h4>The distribution of the LM1 and JIM20 extensin epitopes is restricted to the lining of carinal canals. The monoclonal antibodies JIM5 and LM19 directed against homogalacturonan with a low degree of methyl esterification and the CBM3a probe recognizi2011-01-01T00:00:00Z2011 Aug2025-04-04T12:00:51.691Z2019-03-27T03:07:26ZS-EPMC31430552175279310.1093/aob/mcr161