biostudies-literatureSinsimer KSNCI NIH HHSNIGMS NIH HHS3431-40https://www.ebi.ac.uk/biostudies/studies/S-EPMC3143563biostudies-literatureUnknown138(16)Asymmetric mRNA localization is an effective mechanism for establishing cellular and developmental polarity. Posterior localization of oskar in the Drosophila oocyte targets the synthesis of Oskar to the posterior, where Oskar initiates the assembly of the germ plasm. In addition to harboring germline determinants, the germ plasm is required for localization and translation of the abdominal determinant nanos. Consequently, failure of oskar localization during oogenesis results in embryos lacking germ cells and abdominal segments. oskar accumulates at the oocyte posterior during mid-oogenesis through a well-studied process involving kinesin-mediated transport. Through live imaging of oskar mRNA, we have uncovered a second, mechanistically distinct phase of oskar localization that occurs durDevelopment (Cambridge, England)A late phase of germ plasm accumulation during Drosophila oogenesis requires lost and rumpelstiltskin.PMC3143563T32CA09528F32GM087005F32 GM087005T32 CA009528R01GM067758R01 GM067758Sinsimer KSChatterjee SJain RAGavis ERfalseA late phase of germ plasm accumulation during Drosophila oogenesis requires lost and rumpelstiltskin.Asymmetric mRNA localization is an effective mechanism for establishing cellular and developmental polarity. Posterior localization of oskar in the Drosophila oocyte targets the synthesis of Oskar to the posterior, where Oskar initiates the assembly of the germ plasm. In addition to harboring germline determinants, the germ plasm is required for localization and translation of the abdominal determinant nanos. Consequently, failure of oskar localization during oogenesis results in embryos lacking germ cells and abdominal segments. oskar accumulates at the oocyte posterior during mid-oogenesis through a well-studied process involving kinesin-mediated transport. Through live imaging of oskar mRNA, we have uncovered a second, mechanistically distinct phase of oskar localization that occurs dur2011-01-01T00:00:00Z2011 Aug2025-04-04T12:00:41.793Z2019-03-27T03:07:23ZS-EPMC31435632175293310.1242/dev.065029