{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["108(31)"],"submitter":["Khandelia P"],"pubmed_abstract":["Sequence-specific gene silencing by short hairpin (sh) RNAs has recently emerged as an indispensable tool for understanding gene function and a promising avenue for drug discovery. However, a wider biomedical use of this approach is hindered by the lack of straightforward methods for achieving uniform expression of shRNAs in mammalian cell cultures. Here we report a high-efficiency and low-background (HILO) recombination-mediated cassette exchange (RMCE) technology that yields virtually homogeneous cell pools containing doxycycline-inducible shRNA elements in a matter of days and with minimal efforts. To ensure immediate utility of this approach for a wider research community, we modified 11 commonly used human (A549, HT1080, HEK293T, HeLa, HeLa-S3, and U2OS) and mouse (CAD, L929, N2a, NIH"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pagination":["12799-804"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3150880"],"repository":["biostudies-literature"],"pubmed_title":["Streamlined platform for short hairpin RNA interference and transgenesis in cultured mammalian cells."],"pmcid":["PMC3150880"],"pubmed_authors":["Yap K","Khandelia P","Makeyev EV"],"additional_accession":[]},"is_claimable":false,"name":"Streamlined platform for short hairpin RNA interference and transgenesis in cultured mammalian cells.","description":"Sequence-specific gene silencing by short hairpin (sh) RNAs has recently emerged as an indispensable tool for understanding gene function and a promising avenue for drug discovery. However, a wider biomedical use of this approach is hindered by the lack of straightforward methods for achieving uniform expression of shRNAs in mammalian cell cultures. Here we report a high-efficiency and low-background (HILO) recombination-mediated cassette exchange (RMCE) technology that yields virtually homogeneous cell pools containing doxycycline-inducible shRNA elements in a matter of days and with minimal efforts. To ensure immediate utility of this approach for a wider research community, we modified 11 commonly used human (A549, HT1080, HEK293T, HeLa, HeLa-S3, and U2OS) and mouse (CAD, L929, N2a, NIH","dates":{"release":"2011-01-01T00:00:00Z","publication":"2011 Aug","modification":"2025-04-21T13:47:16.656Z","creation":"2019-03-27T03:07:47Z"},"accession":"S-EPMC3150880","cross_references":{"pubmed":["21768390"],"doi":["10.1073/pnas.1103532108"]}}