biostudies-literatureOh SSNIBIB NIH HHSNHLBI NIH HHS6883-9https://www.ebi.ac.uk/biostudies/studies/S-EPMC3165111biostudies-literatureUnknown83(17)The generation of nucleic acid aptamers with high affinity typically entails a time-consuming, iterative process of binding, separation, and amplification. It would therefore be beneficial to develop an efficient selection strategy that can generate these high-quality aptamers rapidly, economically, and reproducibly. Toward this goal, we have developed a method that efficiently generates DNA aptamers with slow off-rates. This methodology, called VDC-MSELEX, pairs the volume dilution challenge process with microfluidic separation for magnetic bead-assisted aptamer selection. This method offers improved aptamer selection efficiencies through the application of highly stringent selection conditions: it retrieves a small number (<10(6)) of magnetic beads suspended in a large volume (>50 mL) and concentrates them into a microfluidic chamber (8 ?L) with minimal loss for continuous washing. We performed three rounds of the VDC-MSELEX using streptavidin (SA) as the target and obtained new DNA aptamer sequences with low nanomolar affinity that specifically bind to the SA proteins.Analytical chemistryImproving aptamer selection efficiency through volume dilution, magnetic concentration, and continuous washing in microfluidic channels.PMC3165111R01 EB009764-04R01 EB009764U01 HL099773Ahmad KMKim SCho MXiao YOh SSSoh HTfalseImproving aptamer selection efficiency through volume dilution, magnetic concentration, and continuous washing in microfluidic channels.The generation of nucleic acid aptamers with high affinity typically entails a time-consuming, iterative process of binding, separation, and amplification. It would therefore be beneficial to develop an efficient selection strategy that can generate these high-quality aptamers rapidly, economically, and reproducibly. Toward this goal, we have developed a method that efficiently generates DNA aptamers with slow off-rates. This methodology, called VDC-MSELEX, pairs the volume dilution challenge process with microfluidic separation for magnetic bead-assisted aptamer selection. This method offers improved aptamer selection efficiencies through the application of highly stringent selection conditions: it retrieves a small number (<10(6)) of magnetic beads suspended in a large volume (>50 mL) and concentrates them into a microfluidic chamber (8 ?L) with minimal loss for continuous washing. We performed three rounds of the VDC-MSELEX using streptavidin (SA) as the target and obtained new DNA aptamer sequences with low nanomolar affinity that specifically bind to the SA proteins.2011-01-01T00:00:00Z2011 Sep2020-10-29T13:23:09Z2019-03-27T00:43:33ZS-EPMC31651112177445310.1021/ac201269f