{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lopez JE"],"funding":["NHLBI NIH HHS"],"pagination":["629-38"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3166391"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["109(6)"],"pubmed_abstract":["Induction of the fetal hypertrophic marker gene ?-myosin heavy chain (?-MyHC) is a signature feature of pressure overload hypertrophy in rodents. ?-MyHC is assumed present in all or most enlarged myocytes.To quantify the number and size of myocytes expressing endogenous ?-MyHC by a flow cytometry approach.Myocytes were isolated from the left ventricle of male C57BL/6J mice after transverse aortic constriction (TAC), and the fraction of cells expressing endogenous ?-MyHC was quantified by flow cytometry on 10,000 to 20,000 myocytes with use of a validated ?-MyHC antibody. Side scatter by flow cytometry in the same cells was validated as an index of myocyte size. ?-MyHC-positive myocytes constituted 3 ± 1% of myocytes in control hearts (n=12), increasing to 25 ± 10% at 3 days to 6 weeks after TAC (n=24, P<0.01). ?-MyHC-positive myocytes did not enlarge with TAC and were smaller at all times than myocytes without ?-MyHC (?70% as large, P<0.001). ?-MyHC-positive myocytes arose by addition of ?-MyHC to ?-MyHC and had more total MyHC after TAC than did the hypertrophied myocytes that had ?-MyHC only. Myocytes positive for ?-MyHC were found in discrete regions of the left ventricle in 3 patterns: perivascular, in areas with fibrosis, and in apparently normal myocardium.?-MyHC protein is induced by pressure overload in a minor subpopulation of smaller cardiac myocytes. The hypertrophied myocytes after TAC have ?-MyHC only. These data challenge the current paradigm of the fetal hypertrophic gene program and identify a new subpopulation of smaller working ventricular myocytes with more myosin."],"journal":["Circulation research"],"pubmed_title":["?-myosin heavy chain is induced by pressure overload in a minor subpopulation of smaller mouse cardiac myocytes."],"pmcid":["PMC3166391"],"funding_grant_id":["R01 HL031113-21W1","R01 HL031113"],"pubmed_authors":["Swigart PM","Bigos M","Myagmar BE","Rodrigo MC","Lopez JE","Haynam S","Montgomery MD","Simpson PC"],"additional_accession":[]},"is_claimable":false,"name":"?-myosin heavy chain is induced by pressure overload in a minor subpopulation of smaller mouse cardiac myocytes.","description":"Induction of the fetal hypertrophic marker gene ?-myosin heavy chain (?-MyHC) is a signature feature of pressure overload hypertrophy in rodents. ?-MyHC is assumed present in all or most enlarged myocytes.To quantify the number and size of myocytes expressing endogenous ?-MyHC by a flow cytometry approach.Myocytes were isolated from the left ventricle of male C57BL/6J mice after transverse aortic constriction (TAC), and the fraction of cells expressing endogenous ?-MyHC was quantified by flow cytometry on 10,000 to 20,000 myocytes with use of a validated ?-MyHC antibody. Side scatter by flow cytometry in the same cells was validated as an index of myocyte size. ?-MyHC-positive myocytes constituted 3 ± 1% of myocytes in control hearts (n=12), increasing to 25 ± 10% at 3 days to 6 weeks after TAC (n=24, P<0.01). ?-MyHC-positive myocytes did not enlarge with TAC and were smaller at all times than myocytes without ?-MyHC (?70% as large, P<0.001). ?-MyHC-positive myocytes arose by addition of ?-MyHC to ?-MyHC and had more total MyHC after TAC than did the hypertrophied myocytes that had ?-MyHC only. Myocytes positive for ?-MyHC were found in discrete regions of the left ventricle in 3 patterns: perivascular, in areas with fibrosis, and in apparently normal myocardium.?-MyHC protein is induced by pressure overload in a minor subpopulation of smaller cardiac myocytes. The hypertrophied myocytes after TAC have ?-MyHC only. These data challenge the current paradigm of the fetal hypertrophic gene program and identify a new subpopulation of smaller working ventricular myocytes with more myosin.","dates":{"release":"2011-01-01T00:00:00Z","publication":"2011 Sep","modification":"2020-10-29T13:23:49Z","creation":"2019-03-27T00:43:37Z"},"accession":"S-EPMC3166391","cross_references":{"pubmed":["21778428"],"doi":["10.1161/CIRCRESAHA.111.243410","10.1161/circresaha.111.243410"]}}