{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Christensen J"],"funding":["NIAID NIH HHS","NIGMS NIH HHS"],"pagination":["1022-6"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3169396"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["67(Pt 9)"],"pubmed_abstract":["The MPCS Plug Maker is a microcapillary-based protein-crystallization system for generating diffraction-ready crystals from nanovolumes of protein. Crystallization screening using the Plug Maker was used as a salvage pathway for proteins that failed to crystallize during the initial observation period using the traditional sitting-drop vapor-diffusion method. Furthermore, the CrystalCards used to store the crystallization experiments set up by the Plug Maker are shown be a viable container for long-term storage of protein crystals without a discernable loss of diffraction quality with time. Use of the Plug Maker with SSGCID proteins is demonstrated to be an effective crystal-salvage and storage method."],"journal":["Acta crystallographica. Section F, Structural biology and crystallization communications"],"pubmed_title":["Salvage and storage of infectious disease protein targets in the SSGCID high-throughput crystallization pathway using microfluidics."],"pmcid":["PMC3169396"],"funding_grant_id":["U54 GM074961","HHSN272200700057C"],"pubmed_authors":["Christensen J","Clifton MC","Stewart L","Gerdts CJ"],"additional_accession":[]},"is_claimable":false,"name":"Salvage and storage of infectious disease protein targets in the SSGCID high-throughput crystallization pathway using microfluidics.","description":"The MPCS Plug Maker is a microcapillary-based protein-crystallization system for generating diffraction-ready crystals from nanovolumes of protein. Crystallization screening using the Plug Maker was used as a salvage pathway for proteins that failed to crystallize during the initial observation period using the traditional sitting-drop vapor-diffusion method. Furthermore, the CrystalCards used to store the crystallization experiments set up by the Plug Maker are shown be a viable container for long-term storage of protein crystals without a discernable loss of diffraction quality with time. Use of the Plug Maker with SSGCID proteins is demonstrated to be an effective crystal-salvage and storage method.","dates":{"release":"2011-01-01T00:00:00Z","publication":"2011 Sep","modification":"2025-05-29T21:09:52.389Z","creation":"2019-03-27T00:01:06Z"},"accession":"S-EPMC3169396","cross_references":{"pubmed":["21904044"],"doi":["10.1107/S1744309111023232"]}}