biostudies-literatureChristensen JNIAID NIH HHSNIGMS NIH HHS1022-6https://www.ebi.ac.uk/biostudies/studies/S-EPMC3169396biostudies-literatureUnknown67(Pt 9)The MPCS Plug Maker is a microcapillary-based protein-crystallization system for generating diffraction-ready crystals from nanovolumes of protein. Crystallization screening using the Plug Maker was used as a salvage pathway for proteins that failed to crystallize during the initial observation period using the traditional sitting-drop vapor-diffusion method. Furthermore, the CrystalCards used to store the crystallization experiments set up by the Plug Maker are shown be a viable container for long-term storage of protein crystals without a discernable loss of diffraction quality with time. Use of the Plug Maker with SSGCID proteins is demonstrated to be an effective crystal-salvage and storage method.Acta crystallographica. Section F, Structural biology and crystallization communicationsSalvage and storage of infectious disease protein targets in the SSGCID high-throughput crystallization pathway using microfluidics.PMC3169396U54 GM074961HHSN272200700057CChristensen JClifton MCStewart LGerdts CJfalseSalvage and storage of infectious disease protein targets in the SSGCID high-throughput crystallization pathway using microfluidics.The MPCS Plug Maker is a microcapillary-based protein-crystallization system for generating diffraction-ready crystals from nanovolumes of protein. Crystallization screening using the Plug Maker was used as a salvage pathway for proteins that failed to crystallize during the initial observation period using the traditional sitting-drop vapor-diffusion method. Furthermore, the CrystalCards used to store the crystallization experiments set up by the Plug Maker are shown be a viable container for long-term storage of protein crystals without a discernable loss of diffraction quality with time. Use of the Plug Maker with SSGCID proteins is demonstrated to be an effective crystal-salvage and storage method.2011-01-01T00:00:00Z2011 Sep2025-05-29T21:09:52.389Z2019-03-27T00:01:06ZS-EPMC31693962190404410.1107/S1744309111023232