biostudies-literatureLane SWNIDDK NIH HHSNHLBI NIH HHSNCI NIH HHS2849-56https://www.ebi.ac.uk/biostudies/studies/S-EPMC3172801biostudies-literatureUnknown118(10)Hematopoietic stem cells (HSCs) engage in complex bidirectional signals with the hematopoietic microenvironment (HM), and there is emerging evidence that leukemia stem cells (LSCs) may use similar interactions. Using a syngeneic retroviral model of MLL-AF9 induced acute myeloid leukemia (AML), we have identified 2 different stages of leukemia progression, propagated by "pre-LSCs" and established leukemia (LSCs) and compared the homing properties of these distinctive entities to that of normal HSCs. The homing and microlocalization of pre-LSCs was most similar to long-term HSCs and was dependent on cell-intrinsic Wnt signaling. In contrast, the homing of established LSCs was most similar to that of committed myeloid progenitors and distinct from HSCs. Although osteoblast-derived Dickkopf-1,BloodDifferential niche and Wnt requirements during acute myeloid leukemia progression.PMC3172801R01 DK062757P01 CA066996R01 HL044851DK62757HL44851CA66996Lane SWSykes SMBullinger LFerraro FLo Celso CWang YJArmstrong SAWilliams DARagu CShterental SLin CPScadden DTGilliland DGfalseDifferential niche and Wnt requirements during acute myeloid leukemia progression.Hematopoietic stem cells (HSCs) engage in complex bidirectional signals with the hematopoietic microenvironment (HM), and there is emerging evidence that leukemia stem cells (LSCs) may use similar interactions. Using a syngeneic retroviral model of MLL-AF9 induced acute myeloid leukemia (AML), we have identified 2 different stages of leukemia progression, propagated by "pre-LSCs" and established leukemia (LSCs) and compared the homing properties of these distinctive entities to that of normal HSCs. The homing and microlocalization of pre-LSCs was most similar to long-term HSCs and was dependent on cell-intrinsic Wnt signaling. In contrast, the homing of established LSCs was most similar to that of committed myeloid progenitors and distinct from HSCs. Although osteoblast-derived Dickkopf-1,2011-01-01T00:00:00Z2011 Sep2025-07-02T03:04:57.89Z2025-07-02T03:04:57.89ZS-EPMC31728012176502110.1182/blood-2011-03-345165