biostudies-literatureLi RCNHLBI NIH HHS124-31https://www.ebi.ac.uk/biostudies/studies/S-EPMC3172891biostudies-literatureUnknown184(1)<h4>Rationale</h4>Obstructive sleep apnea, which is characterized by intermittent hypoxia (IH) during sleep, has emerged as an independent risk factor for cardiovascular disease, including atherosclerosis. Leukotriene B4 (LTB4) production is increased in patients with obstructive sleep apnea and negatively correlates to hypoxic levels during sleep, with continuous positive airway pressure therapy decreasing LTB4 production.<h4>Objectives</h4>Determine the potential role of LTB4 in IH-induced atherosclerosis in a monocyte cellular model and a murine model.<h4>Methods</h4>THP-1 cells were exposed to IH for 3, 6, 24, and 48 hours. Macrophage transformation and foam cell formation were assessed after IH exposures. Apolipopotein E (ApoE)(-/-) or BLT1(-/-)/ApoE(-/-) mice were fed an atherogenic American journal of respiratory and critical care medicineLeukotriene B4 receptor-1 mediates intermittent hypoxia-induced atherogenesis.PMC3172891R01 HL065270HL-086662HL-65270R01 HL086662Mathis SPGozal DLi RCKim JHaribabu BfalseLeukotriene B4 receptor-1 mediates intermittent hypoxia-induced atherogenesis.<h4>Rationale</h4>Obstructive sleep apnea, which is characterized by intermittent hypoxia (IH) during sleep, has emerged as an independent risk factor for cardiovascular disease, including atherosclerosis. Leukotriene B4 (LTB4) production is increased in patients with obstructive sleep apnea and negatively correlates to hypoxic levels during sleep, with continuous positive airway pressure therapy decreasing LTB4 production.<h4>Objectives</h4>Determine the potential role of LTB4 in IH-induced atherosclerosis in a monocyte cellular model and a murine model.<h4>Methods</h4>THP-1 cells were exposed to IH for 3, 6, 24, and 48 hours. Macrophage transformation and foam cell formation were assessed after IH exposures. Apolipopotein E (ApoE)(-/-) or BLT1(-/-)/ApoE(-/-) mice were fed an atherogenic 2011-01-01T00:00:00Z2011 Jul2025-04-18T13:21:50.923Z2019-03-27T00:43:55ZS-EPMC31728912149373510.1164/rccm.201012-2039OC10.1164/rccm.201012-2039oc