{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["39(17)"],"submitter":["Pillet F"],"pubmed_abstract":["The segregation of plasmid F of Escherichia coli is highly reliable. The Sop partition locus, responsible for this stable maintenance, is composed of two genes, sopA and sopB and a centromere, sopC, consisting of 12 direct repeats of 43 bp. Each repeat carries a 16-bp inverted repeat motif to which SopB binds to form a nucleoprotein assembly called the partition complex. A database search for sequences closely related to sopC revealed unexpected features that appeared highly conserved. We have investigated the requirements for specific SopB-sopC interactions using a surface plasmon resonance imaging technique. We show that (i) only 10 repeats interact specifically with SopB, (ii) no base outside the 16-bp sopC sites is involved in binding specificity, whereas five bases present in each arm"],"journal":["Nucleic acids research"],"pagination":["7477-86"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3177203"],"repository":["biostudies-literature"],"pubmed_title":["Centromere binding specificity in assembly of the F plasmid partition complex."],"pmcid":["PMC3177203"],"pubmed_authors":["Sanchez A","Bouet JY","Pillet F","Lane D","Anton Leberre V"],"additional_accession":[]},"is_claimable":false,"name":"Centromere binding specificity in assembly of the F plasmid partition complex.","description":"The segregation of plasmid F of Escherichia coli is highly reliable. The Sop partition locus, responsible for this stable maintenance, is composed of two genes, sopA and sopB and a centromere, sopC, consisting of 12 direct repeats of 43 bp. Each repeat carries a 16-bp inverted repeat motif to which SopB binds to form a nucleoprotein assembly called the partition complex. A database search for sequences closely related to sopC revealed unexpected features that appeared highly conserved. We have investigated the requirements for specific SopB-sopC interactions using a surface plasmon resonance imaging technique. We show that (i) only 10 repeats interact specifically with SopB, (ii) no base outside the 16-bp sopC sites is involved in binding specificity, whereas five bases present in each arm","dates":{"release":"2011-01-01T00:00:00Z","publication":"2011 Sep","modification":"2025-04-05T10:23:46.678Z","creation":"2019-03-27T00:44:09Z"},"accession":"S-EPMC3177203","cross_references":{"pubmed":["21653553"],"doi":["10.1093/nar/gkr457"]}}