biostudies-literatureUnknown6Aksaas AK<h4>Background</h4>Post-transcriptional processing of pre-mRNA takes place in several steps and requires involvement of a number of RNA-binding proteins. How pre-mRNA processing is regulated is in large enigmatic. The catalytic (C) subunit of protein kinase A (PKA) is a serine/threonine kinase, which regulates numerous cellular processes including pre-mRNA splicing. Despite that a significant fraction of the C subunit is found in splicing factor compartments in the nucleus, there are no indications of a direct interaction between RNA and PKA. Based on this we speculate if the specificity of the C subunit in regulating pre-mRNA splicing may be mediated indirectly through other nuclear proteins.<h4>Results</h4>Using yeast two-hybrid screening with the PKA C subunit Cbeta2 as bait, we identifJournal of molecular signaling10https://www.ebi.ac.uk/biostudies/studies/S-EPMC3179746biostudies-literatureG-patch domain and KOW motifs-containing protein, GPKOW; a nuclear RNA-binding protein regulated by protein kinase A.PMC3179746Kvissel AKSkalhegg BSRogne MRosendal KLarsen ACAksaas AKfalseG-patch domain and KOW motifs-containing protein, GPKOW; a nuclear RNA-binding protein regulated by protein kinase A.<h4>Background</h4>Post-transcriptional processing of pre-mRNA takes place in several steps and requires involvement of a number of RNA-binding proteins. How pre-mRNA processing is regulated is in large enigmatic. The catalytic (C) subunit of protein kinase A (PKA) is a serine/threonine kinase, which regulates numerous cellular processes including pre-mRNA splicing. Despite that a significant fraction of the C subunit is found in splicing factor compartments in the nucleus, there are no indications of a direct interaction between RNA and PKA. Based on this we speculate if the specificity of the C subunit in regulating pre-mRNA splicing may be mediated indirectly through other nuclear proteins.<h4>Results</h4>Using yeast two-hybrid screening with the PKA C subunit Cbeta2 as bait, we identif2011-01-01T00:00:00Z2011 Aug2026-04-07T14:10:43.251Z2025-07-09T03:05:16.281ZS-EPMC31797462188014210.1186/1750-2187-6-10