biostudies-literatureDowal LNHLBI NIH HHSe62-73https://www.ebi.ac.uk/biostudies/studies/S-EPMC3186346biostudies-literatureUnknown118(13)Protein palmitoylation is a dynamic process that regulates membrane targeting of proteins and protein-protein interactions. We have previously demonstrated a critical role for protein palmitoylation in platelet activation and have identified palmitoylation machinery in platelets. Using a novel proteomic approach, Palmitoyl Protein Identification and Site Characterization, we have begun to characterize the human platelet palmitoylome. Palmitoylated proteins were enriched from membranes isolated from resting platelets using acyl-biotinyl exchange chemistry, followed by identification using liquid chromatography-tandem mass spectrometry. This global analysis identified > 1300 proteins, of which 215 met criteria for significance and represent the platelet palmitoylome. This collection includesBloodProteomic analysis of palmitoylated platelet proteins.PMC3186346T32 HL07917T32 HL007917HL87203P01 HL087203Yang WFlaumenhaft RFreeman MRSteen HDowal LfalseProteomic analysis of palmitoylated platelet proteins.Protein palmitoylation is a dynamic process that regulates membrane targeting of proteins and protein-protein interactions. We have previously demonstrated a critical role for protein palmitoylation in platelet activation and have identified palmitoylation machinery in platelets. Using a novel proteomic approach, Palmitoyl Protein Identification and Site Characterization, we have begun to characterize the human platelet palmitoylome. Palmitoylated proteins were enriched from membranes isolated from resting platelets using acyl-biotinyl exchange chemistry, followed by identification using liquid chromatography-tandem mass spectrometry. This global analysis identified > 1300 proteins, of which 215 met criteria for significance and represent the platelet palmitoylome. This collection includes2011-01-01T00:00:00Z2011 Sep2026-06-03T10:52:10.485Z2026-04-28T03:11:04.814ZS-EPMC31863462181344910.1182/blood-2011-05-353078