biostudies-literatureUnknown12(9)Wu MJThis study was carried out with fresh Australian lager beer which was sampled directly off the production line, the same samples aged for 12 weeks at 30 °C, and the vintage beer which was kept at 20 °C for 5 years. Characteristic Australian lager flavour was maintained in the fresh and vintage beers but was lost in the aged beer. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and free thiol group labelling analyses of beer proteins found that this flavour stability correlated with the presence of an unknown 10 kilodaltons (kDa) protein with a higher level of free thiols. The protein was purified by size-exclusion chromatography, then peptide sequencing and database matching identified it as the barley lipid transfer protein (LTP1). Further characterisation using diphInternational journal of molecular sciences6089-103https://www.ebi.ac.uk/biostudies/studies/S-EPMC3189770biostudies-literatureIdentification of a protein with antioxidant activity that is important for the protection against beer ageing.PMC3189770Wu MJYoung PHiggins VJSales NRogers PJO'Doherty PJClarke FMfalseIdentification of a protein with antioxidant activity that is important for the protection against beer ageing.This study was carried out with fresh Australian lager beer which was sampled directly off the production line, the same samples aged for 12 weeks at 30 °C, and the vintage beer which was kept at 20 °C for 5 years. Characteristic Australian lager flavour was maintained in the fresh and vintage beers but was lost in the aged beer. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and free thiol group labelling analyses of beer proteins found that this flavour stability correlated with the presence of an unknown 10 kilodaltons (kDa) protein with a higher level of free thiols. The protein was purified by size-exclusion chromatography, then peptide sequencing and database matching identified it as the barley lipid transfer protein (LTP1). Further characterisation using diph2011-01-01T00:00:00Z20112025-04-19T01:17:40.727Z2019-06-05T16:47:51ZS-EPMC31897702201664610.3390/ijms12096089