{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["22(20)"],"submitter":["Maeda M"],"pubmed_abstract":["Rho GTPases are molecular switches that transmit biochemical signals in response to extracellular stimuli to elicit changes in the actin cytoskeleton. Rho GTPases cycle between an active, GTP-bound state and an inactive, GDP-bound state. These states are regulated by two distinct families of proteins-guanine nucleotide exchange factors and GTPase-activating proteins (GAPs). We studied the role of a previously uncharacterized GAP, ARHGAP18 (MacGAP). Overexpression of ARHGAP18 suppressed the activity of RhoA and disrupted stress fiber formation. Conversely, silencing of ARHGAP18 by small interfering RNA transfection-enhanced stress fiber formation and induced rounding of cells. We examined the role of ARHGAP18 in cell spreading and migration. Immunofluorescence analysis revealed that ARHGAP1"],"journal":["Molecular biology of the cell"],"pagination":["3840-52"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3192863"],"repository":["biostudies-literature"],"pubmed_title":["ARHGAP18, a GTPase-activating protein for RhoA, controls cell shape, spreading, and motility."],"pmcid":["PMC3192863"],"pubmed_authors":["Shimokata K","Hasegawa H","Senga T","Asano E","Yuang H","Hyodo T","Ito S","Hasegawa Y","Funasaka K","Hamaguchi M","Maeda M"],"additional_accession":[]},"is_claimable":false,"name":"ARHGAP18, a GTPase-activating protein for RhoA, controls cell shape, spreading, and motility.","description":"Rho GTPases are molecular switches that transmit biochemical signals in response to extracellular stimuli to elicit changes in the actin cytoskeleton. Rho GTPases cycle between an active, GTP-bound state and an inactive, GDP-bound state. These states are regulated by two distinct families of proteins-guanine nucleotide exchange factors and GTPase-activating proteins (GAPs). We studied the role of a previously uncharacterized GAP, ARHGAP18 (MacGAP). Overexpression of ARHGAP18 suppressed the activity of RhoA and disrupted stress fiber formation. Conversely, silencing of ARHGAP18 by small interfering RNA transfection-enhanced stress fiber formation and induced rounding of cells. We examined the role of ARHGAP18 in cell spreading and migration. Immunofluorescence analysis revealed that ARHGAP1","dates":{"release":"2011-01-01T00:00:00Z","publication":"2011 Oct","modification":"2025-04-04T07:24:43.534Z","creation":"2019-03-27T00:44:56Z"},"accession":"S-EPMC3192863","cross_references":{"pubmed":["21865595"],"doi":["10.1091/mbc.E11-04-0364"]}}