biostudies-literatureUnknown11Kovacs-Bogdan E<h4>Background</h4>The Tic complex (Translocon at the inner envelope membrane of chloroplasts) mediates the translocation of nuclear encoded chloroplast proteins across the inner envelope membrane. Tic110 forms one prominent protein translocation channel. Additionally, Tic20, another subunit of the complex, was proposed to form a protein import channel - either together with or independent of Tic110. However, no experimental evidence for Tic20 channel activity has been provided so far.<h4>Results</h4>We performed a comprehensive biochemical and electrophysiological study to characterize Tic20 in more detail and to gain a deeper insight into its potential role in protein import into chloroplasts. Firstly, we compared transcript and protein levels of Tic20 and Tic110 in both Pisum sativum anBMC plant biology133https://www.ebi.ac.uk/biostudies/studies/S-EPMC3203047biostudies-literatureTic20 forms a channel independent of Tic110 in chloroplasts.PMC3203047Soll JKovacs-Bogdan EBolter BBenz JPfalseTic20 forms a channel independent of Tic110 in chloroplasts.<h4>Background</h4>The Tic complex (Translocon at the inner envelope membrane of chloroplasts) mediates the translocation of nuclear encoded chloroplast proteins across the inner envelope membrane. Tic110 forms one prominent protein translocation channel. Additionally, Tic20, another subunit of the complex, was proposed to form a protein import channel - either together with or independent of Tic110. However, no experimental evidence for Tic20 channel activity has been provided so far.<h4>Results</h4>We performed a comprehensive biochemical and electrophysiological study to characterize Tic20 in more detail and to gain a deeper insight into its potential role in protein import into chloroplasts. Firstly, we compared transcript and protein levels of Tic20 and Tic110 in both Pisum sativum an2011-01-01T00:00:00Z2011 Sep2026-04-07T13:51:59.43Z2026-04-07T13:36:19.83ZS-EPMC32030472196152510.1186/1471-2229-11-133