biostudies-literatureUnknown10(10)Buchse TThe Cbl-interacting 85-kDa protein (CIN85) plays an important role as a negative regulator of signaling pathways induced by receptor tyrosine kinases. By assembling multiprotein complexes this versatile adaptor enhances receptor tyrosine kinase-activated clathrin-mediated endocytosis and reduces phosphatidylinositol-3-kinase-induced phosphatidylinositol-3,4,5-trisphosphate production. Here we report the expression of CIN85 in primary splenic B lymphocytes and the B-lymphoma cell lines WEHI 231 and Ba/F3. Cross-linking of the B cell antigen receptor resulted in an increased association of CIN85 with the ubiquitin ligase Cbl. Through a systematic pull-down proteomics approach we identified 51 proteins that interact with CIN85 in B cells, including proteins not shown previously to be CIN85-asMolecular & cellular proteomics : MCPM110.006239https://www.ebi.ac.uk/biostudies/studies/S-EPMC3205855biostudies-literatureCIN85 interacting proteins in B cells-specific role for SHIP-1.PMC3205855Horras NMikkat SLenfert ETiedge MKorbel SKrystal GKrause ESchumann MBuchse TfalseCIN85 interacting proteins in B cells-specific role for SHIP-1.The Cbl-interacting 85-kDa protein (CIN85) plays an important role as a negative regulator of signaling pathways induced by receptor tyrosine kinases. By assembling multiprotein complexes this versatile adaptor enhances receptor tyrosine kinase-activated clathrin-mediated endocytosis and reduces phosphatidylinositol-3-kinase-induced phosphatidylinositol-3,4,5-trisphosphate production. Here we report the expression of CIN85 in primary splenic B lymphocytes and the B-lymphoma cell lines WEHI 231 and Ba/F3. Cross-linking of the B cell antigen receptor resulted in an increased association of CIN85 with the ubiquitin ligase Cbl. Through a systematic pull-down proteomics approach we identified 51 proteins that interact with CIN85 in B cells, including proteins not shown previously to be CIN85-as2011-01-01T00:00:00Z2011 Oct2025-04-18T17:24:56.302Z2019-06-05T16:51:31ZS-EPMC32058552172506110.1074/mcp.m110.00623910.1074/mcp.M110.006239