{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["6(11)"],"submitter":["Pedro L"],"pubmed_abstract":["The nucleoid-associated proteins Hha and YdgT repress the expression of the toxin α-hemolysin. An Escherichia coli mutant lacking these proteins overexpresses the toxin α-hemolysin encoded in the multicopy recombinant plasmid pANN202-312R. Unexpectedly, we could observe that this mutant generated clones that no further produced hemolysin (Hly(-)). Generation of Hly(-) clones was dependent upon the presence in the culture medium of the antibiotic kanamycin (km), a marker of the hha allele (hha::Tn5). Detailed analysis of different Hly(-) clones evidenced that recombination between partial IS91 sequences that flank the hly operon had occurred. A fluctuation test evidenced that the presence of km in the culture medium was underlying the generation of these clones. A decrease of the km concent"],"journal":["PloS one"],"pagination":["e27606"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3214074"],"repository":["biostudies-literature"],"pubmed_title":["Antibiotics shaping bacterial genome: deletion of an IS91 flanked virulence determinant upon exposure to subinhibitory antibiotic concentrations."],"pmcid":["PMC3214074"],"pubmed_authors":["Madrid C","Aznar S","Pedro L","Banos RC","Juarez A","Balsalobre C"],"additional_accession":[]},"is_claimable":false,"name":"Antibiotics shaping bacterial genome: deletion of an IS91 flanked virulence determinant upon exposure to subinhibitory antibiotic concentrations.","description":"The nucleoid-associated proteins Hha and YdgT repress the expression of the toxin α-hemolysin. An Escherichia coli mutant lacking these proteins overexpresses the toxin α-hemolysin encoded in the multicopy recombinant plasmid pANN202-312R. Unexpectedly, we could observe that this mutant generated clones that no further produced hemolysin (Hly(-)). Generation of Hly(-) clones was dependent upon the presence in the culture medium of the antibiotic kanamycin (km), a marker of the hha allele (hha::Tn5). Detailed analysis of different Hly(-) clones evidenced that recombination between partial IS91 sequences that flank the hly operon had occurred. A fluctuation test evidenced that the presence of km in the culture medium was underlying the generation of these clones. A decrease of the km concent","dates":{"release":"2011-01-01T00:00:00Z","publication":"2011","modification":"2026-04-08T00:53:28.471Z","creation":"2025-07-09T03:05:11.382Z"},"accession":"S-EPMC3214074","cross_references":{"pubmed":["22096603"],"doi":["10.1371/journal.pone.0027606"]}}