{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["7(12)"],"submitter":["Cimino PA"],"pubmed_abstract":["Translational readthrough of stop codons by ribosomes is a recoding event used by a variety of viruses, including plus-strand RNA tombusviruses. Translation of the viral RNA-dependent RNA polymerase (RdRp) in tombusviruses is mediated using this strategy and we have investigated this process using a variety of in vitro and in vivo approaches. Our results indicate that readthrough generating the RdRp requires a novel long-range RNA-RNA interaction, spanning a distance of ∼3.5 kb, which occurs between a large RNA stem-loop located 3'-proximal to the stop codon and an RNA replication structure termed RIV at the 3'-end of the viral genome. Interestingly, this long-distance RNA-RNA interaction is modulated by mutually-exclusive RNA structures in RIV that represent a type of RNA switch. Moreover"],"journal":["PLoS pathogens"],"pagination":["e1002423"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3234231"],"repository":["biostudies-literature"],"pubmed_title":["Multifaceted regulation of translational readthrough by RNA replication elements in a tombusvirus."],"pmcid":["PMC3234231"],"pubmed_authors":["White KA","Nicholson BL","Cimino PA","Wu B","Xu W"],"additional_accession":[]},"is_claimable":false,"name":"Multifaceted regulation of translational readthrough by RNA replication elements in a tombusvirus.","description":"Translational readthrough of stop codons by ribosomes is a recoding event used by a variety of viruses, including plus-strand RNA tombusviruses. Translation of the viral RNA-dependent RNA polymerase (RdRp) in tombusviruses is mediated using this strategy and we have investigated this process using a variety of in vitro and in vivo approaches. Our results indicate that readthrough generating the RdRp requires a novel long-range RNA-RNA interaction, spanning a distance of ∼3.5 kb, which occurs between a large RNA stem-loop located 3'-proximal to the stop codon and an RNA replication structure termed RIV at the 3'-end of the viral genome. Interestingly, this long-distance RNA-RNA interaction is modulated by mutually-exclusive RNA structures in RIV that represent a type of RNA switch. Moreover","dates":{"release":"2011-01-01T00:00:00Z","publication":"2011 Dec","modification":"2026-04-07T18:55:48.041Z","creation":"2019-03-27T00:46:51Z"},"accession":"S-EPMC3234231","cross_references":{"pubmed":["22174683"],"doi":["10.1371/journal.ppat.1002423"]}}