{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(12)"],"submitter":["Maiuri P"],"pubmed_abstract":["Averaged estimates of RNA polymerase II (RNAPII) elongation rates in mammalian cells have been shown to range between 1.3 and 4.3 kb min(-1). In this work, nascent RNAs from an integrated human immunodeficiency virus type 1-derived vector were detectable at the single living cell level by fluorescent RNA tagging. At steady state, a constant number of RNAs was measured corresponding to a minimal density of polymerases with negligible fluctuations over time. Recovery of fluorescence after photobleaching was complete within seconds, indicating a high rate of RNA biogenesis. The calculated transcription rate above 50 kb min(-1) points towards a wide dynamic range of RNAPII velocities in living cells."],"journal":["EMBO reports"],"pagination":["1280-5"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3245692"],"repository":["biostudies-literature"],"pubmed_title":["Fast transcription rates of RNA polymerase II in human cells."],"pmcid":["PMC3245692"],"pubmed_authors":["Marcello A","Knezevich A","Mazza D","McNally JG","Maiuri P","De Marco A","Kula A"],"additional_accession":[]},"is_claimable":false,"name":"Fast transcription rates of RNA polymerase II in human cells.","description":"Averaged estimates of RNA polymerase II (RNAPII) elongation rates in mammalian cells have been shown to range between 1.3 and 4.3 kb min(-1). In this work, nascent RNAs from an integrated human immunodeficiency virus type 1-derived vector were detectable at the single living cell level by fluorescent RNA tagging. At steady state, a constant number of RNAs was measured corresponding to a minimal density of polymerases with negligible fluctuations over time. Recovery of fluorescence after photobleaching was complete within seconds, indicating a high rate of RNA biogenesis. The calculated transcription rate above 50 kb min(-1) points towards a wide dynamic range of RNAPII velocities in living cells.","dates":{"release":"2011-01-01T00:00:00Z","publication":"2011 Dec","modification":"2025-04-05T00:23:43.969Z","creation":"2019-03-27T00:47:20Z"},"accession":"S-EPMC3245692","cross_references":{"pubmed":["22015688"],"doi":["10.1038/embor.2011.196"]}}