biostudies-literatureOrtiz SNCI NIH HHS1450-63https://www.ebi.ac.uk/biostudies/studies/S-EPMC3247199biostudies-literatureUnknown235(12)Activation of T lymphoma cells expressing Syk, but not ZAP-70 tyrosine kinase, has been shown to negatively regulate cell activation and activation-induced cell death (AICD), perhaps due to differential induction of tyrosine phosphorylation modified proteins. To better understand the role of these proteins and their associated molecules/pathways, we studied a previously described model of T lymphoma cells expressing either a kinase-activated chimeric Syk or ZAP-70 genetically linked to T-cell receptor (TCR) ζ chain (Z/Syk or Z/ZAP cells, respectively). To help identify molecules and pathways linked to cell activation or AICD, a comparative semi-quantitative proteomics-based approach was utilized to analyze tyrosine-phosphorylated protein immunoprecipitates from two-minute short-term activaExperimental biology and medicine (Maywood, N.J.)Comparative analyses of differentially induced T-cell receptor-mediated phosphorylation pathways in T lymphoma cells.PMC3247199P50 CA107399P30 CA033572P30 CA33572F31 CA117055Smith DLiu CPLee TDOrtiz SLee WForman SJfalseComparative analyses of differentially induced T-cell receptor-mediated phosphorylation pathways in T lymphoma cells.Activation of T lymphoma cells expressing Syk, but not ZAP-70 tyrosine kinase, has been shown to negatively regulate cell activation and activation-induced cell death (AICD), perhaps due to differential induction of tyrosine phosphorylation modified proteins. To better understand the role of these proteins and their associated molecules/pathways, we studied a previously described model of T lymphoma cells expressing either a kinase-activated chimeric Syk or ZAP-70 genetically linked to T-cell receptor (TCR) ζ chain (Z/Syk or Z/ZAP cells, respectively). To help identify molecules and pathways linked to cell activation or AICD, a comparative semi-quantitative proteomics-based approach was utilized to analyze tyrosine-phosphorylated protein immunoprecipitates from two-minute short-term activa2010-01-01T00:00:00Z2010 Dec2026-04-20T03:13:51.075Z2026-04-20T03:10:03.847ZS-EPMC32471992112734210.1258/ebm.2010.010056