{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Xiong X"],"funding":["NIDDK NIH HHS","NCI NIH HHS","NIGMS NIH HHS"],"pagination":["87-98"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3248907"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(1)"],"pubmed_abstract":["E-Cadherin-mediated formation of adherens junctions (AJs) is essential for the morphogenesis of epithelial cells. However, the mechanisms underlying E-cadherin clustering and AJ maturation are not fully understood. Here we report that type Iγ phosphatidylinositol-4-phosphate 5-kinase (PIPKIγ) associates with the exocyst via a direct interaction with Exo70, the exocyst subunit that guides the polarized targeting of exocyst to the plasma membrane. By means of this interaction, PIPKIγ mediates the association between E-cadherin and Exo70 and determines the targeting of Exo70 to AJs. Further investigation revealed that Exo70 is necessary for clustering of E-cadherin on the plasma membrane and extension of nascent E-cadherin adhesions, which are critical for the maturation of cohesive AJs. In a"],"journal":["Molecular biology of the cell"],"pubmed_title":["An association between type Iγ PI4P 5-kinase and Exo70 directs E-cadherin clustering and epithelial polarization."],"pmcid":["PMC3248907"],"funding_grant_id":["R01 CA149039","R37 GM055816","R01 DK090038","P30 DK084567","1R01CA149039-01A1"],"pubmed_authors":["Huang Y","Hu J","Leof E","Xu Q","Singh RD","Anderson R","Ling K","Xiong X"],"additional_accession":[]},"is_claimable":false,"name":"An association between type Iγ PI4P 5-kinase and Exo70 directs E-cadherin clustering and epithelial polarization.","description":"E-Cadherin-mediated formation of adherens junctions (AJs) is essential for the morphogenesis of epithelial cells. However, the mechanisms underlying E-cadherin clustering and AJ maturation are not fully understood. Here we report that type Iγ phosphatidylinositol-4-phosphate 5-kinase (PIPKIγ) associates with the exocyst via a direct interaction with Exo70, the exocyst subunit that guides the polarized targeting of exocyst to the plasma membrane. By means of this interaction, PIPKIγ mediates the association between E-cadherin and Exo70 and determines the targeting of Exo70 to AJs. Further investigation revealed that Exo70 is necessary for clustering of E-cadherin on the plasma membrane and extension of nascent E-cadherin adhesions, which are critical for the maturation of cohesive AJs. In a","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 Jan","modification":"2025-04-19T13:48:14.486Z","creation":"2019-03-27T00:47:30Z"},"accession":"S-EPMC3248907","cross_references":{"pubmed":["22049025"],"doi":["10.1091/mbc.E11-05-0449"]}}